毛果杨CBF/DREB1基因家族生物信息学分析  

Bioinformatics Analysis of CBF/DREB1 Family in Populus trichocarpa

作　　者：丁咚 陈亚娟[2] 崔进荣 魏建华[2] 张玉红 张杰伟[2] DING Dong;CHEN Ya-juan;CUI Jin-rong;WEI Jian-hua;ZHANG Yu-hong;ZHANG Jie-wei(Key Laboratory of Ministry of Education in Forestry Plant Ecology,Northeast Forestry University,Heilongjiang Harbin 150040,China;Beijing Agro-Biotechnology Research Center,Beijing Academy of Agriculture and Forestry Sciences;Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology,Beijing 100097,China)

机构地区：[1]东北林业大学,森林植物生态学教育部重点实验室,黑龙江哈尔滨150040 [2]北京市农林科学院北京农业生物技术研究中心,农业基因资源与生物技术北京市重点实验室,北京100097

出　　处：《西南农业学报》2018年第3期457-461,共5页Southwest China Journal of Agricultural Sciences

基　　金：国家“863”项目(2013AA102702);国家自然科学基金(31270593);北京市农林科学院科技创新能力建设专项(KJCX20140202)

摘　　要：【目的】为了对毛果杨CBF基因家族进行系统分析,利用毛果杨基因组数据库,通过生物信息学的方法,鉴定毛果杨CBF基因家族的染色体定位、编码蛋白和磷酸化位点预测。【方法】通过序列比对进行进化和分类分析。【结果】毛果杨含有6个CBF基因,均不含内显子,分布于毛果杨的5条染色体上。【结论】MEME保守基序分析显示,杨树CBF蛋白均含有2个保守的基序,他们共同构成AP2结构域。磷酸化位点预测分析表明毛果杨CBF蛋白均含有大量的磷酸化位点。以上结果将为今后揭示毛果杨CBF蛋白的功能提供重要的线索。【Objective】The study aims to analyze systematically CBF gene family in Populus trichocarpa.【Method】On the basis of P.trichocarpa genome database and bioinformatics method,we obtained P.trichocarpa CBF gene structure,position on chromosome,proteins and phosphorylation site.【Result】Six PtCBF genes existed in the P.trichocarpa genome and located on 5 different chromosomes.【Conclusion】Using MEME method,multiple alignment and motif display results indicated that all PtCBF proteins contain two conserved motifs,constituted AP2 domain.Phylogenetic analysis revealed that the PtCBF proteins could be divided into 3 groups.Phosphorylation site prediction of PtCBF proteins showed that there were many phosphorylation sites.These results would provide the important clues for functional studies to reveal the role of CBF/DREB1 gene family in P.trichocarpa.

关 键 词：杨树 CBF/DREB1 基因家族 进化分析 

分 类 号：Q945.78[生物学—植物学] S601[农业科学—园艺学]