枣离体幼胚发育影响因素的研究  

Study on Factors Affecting Immature Embryo Development of Chinese Jujube

作　　者：任海燕[1,2] 杜学梅 李登科[1] 杜俊杰[2] 王永康[1] 赵爱玲[1] 薛晓芳 弓桂花[1] 王燕平[1] REN Hai-yan;DU Xue-mei;LI Deng-ke;DU Jun-jie;WANG Yong-kang;ZHAO Ai-ling;XUE Xiao-fang;GONG Gui-hua;WANG Yan-ping(Shanxi Provincial Key Laboratory of Genetic Improvement and Use of Pomology,Institute of Pomology,Shanxi Academy of Agricultural Sciences,Shanxi Taigu 030815,China;School of Horticulture,Shanxi Agriculture University,Shanxi Taigu 030801,China)

机构地区：[1]山西省农业科学院果树研究所,果树种质创制与利用山西省重点实验室,山西太谷030815 [2]山西农业大学园艺学院,山西太谷030815

出　　处：《西南农业学报》2018年第3期571-576,共6页Southwest China Journal of Agricultural Sciences

基　　金：国家科技部科技支撑项目(2013BAD14B030101);山西省青年科技研究基金(2015021163);山西省留学办留学基金项目(2012-13);国家农业部农作物种质资源保护项目(NB2014-2130135-02);枣种质资源创新与利用山西省科技创新重点团队;果树种质创制与利用山西省重点实验室

摘　　要：【目的】为延长败育前枣幼胚的发育程度,提高枣幼胚挽救效率。【方法】以枣品种‘冷白玉’、‘六月鲜’、‘襄汾圆枣’胚龄20~40 d幼胚为试材,在明确枣幼胚发育状况的基础上,对影响离体幼胚发育的基因型、适宜取样的最小胚龄、植物生长调节物质进行了研究。【结果】‘冷白玉’和‘六月鲜’枣20~40 d胚龄的幼胚发育相对一致,而‘襄汾圆枣’幼胚发育快于这2个品种;3个品种幼胚成胚难易程度依次为‘冷白玉’、‘襄汾圆枣’、‘六月鲜’;不同枣品种、不同胚龄对植物生长调节物质的响应不同;适宜3个品种幼胚挽救取样的最小取样胚龄为30 d,‘冷白玉’、‘襄汾圆枣’、‘六月鲜’成胚率分别为56.82%、54.17%、52.53%。【结论】胚龄对枣离体幼胚生长发育的影响最大,按作用大小排序依次为胚龄>品种>植物生长调节物质种类>植物生长调节物质浓度。【Objective】The present study aims to improve the development of immature embryos and increase the efficiency of immature embryos of Chinese Jujube.【Method】Three varieties of Chinese jujube(Ziziphus jujuba‘Lengbaiyu’,Ziziphusjujuba‘Liuyuexian’,Ziziphusjujuba‘Xiangfenyuanzao’)in 20 to 40 day sold,known for their relatively less severe embryo abortion,were selected as materials.Based on the observation of the development of the young embryo,the effects of genotypes,the minimum embryonic age appropriate sampling and different hormones on the development of the Chinese jujube were studied.【Result】20-40 days embryos development of‘Lengbaiyu’and‘Liuyuexian’was relatively consistent,and the same embryos development‘Xiangfenyuanzao’was faster than them and immature embryo culture easily followed by‘Lengbaiyu’,‘Xiangfenyuanzao’,‘Liuyuexian’.The different jujube varieties at different embryonic age and young embryo developmental stage,the response to the exogenous hormones were different.The minimum sampling embryonic age of immature embryos for the three varieties were 30 days.The embryogenic rate of‘Lengbaiy u’,‘Xiangfenyuanzao’and‘Liuyuexian’were respectively 56.82%,54.17%52.53%.【Conclusion】The analysis showed that the embryonic age had the greatest effect on the growth and development of juvenile immature embryos,followed by embryo age>varieties>the type of exogenous hormones>the concentration of exogenous hormones.

关 键 词：枣 幼胚发育 基因型 胚龄 植物生长调节物质 

分 类 号：S665.1[农业科学—果树学]