miR-29s通过下调大麻素受体1抑制ACEA促J774A.1细胞迁移的活性  

miR-29s inhibit ACEA-induced J774A.1 cell migration by down regulation CB1 expression

作　　者：段向辉 常娜[1] 李丽英[1] Duan Xianghui;Chang Na;Li Liying(Department of Cell Biology,School of Basic Medical Sciences,Capital Medical University,Municipal Laboratory for Liver Protection and Regulation of Regeneration,Beijing 100069,China)

机构地区：[1]首都医科大学基础医学院细胞生物学系,'肝脏保护与再生调节'北京市重点实验室,北京100069

出　　处：《首都医科大学学报》2018年第2期230-235,共6页Journal of Capital Medical University

基　　金：国家自然科学基金(81670550),北京市属高等学校创新团队建设与教师职业发展计划项目(IDHT20150502)。

摘　　要：目的研究miR-29a-3p、miR-29b-3p和miR-29c-3p是否可以影响J774A.1细胞中大麻素受体1(cannabinoid receptor type 1,CB1)的表达以及其迁移功能。方法 RT-q PCR法检测CB1 mRNA表达;Boyden chamber法检测J774A.1细胞迁移能力的变化。结果本研究证明了在J774A.1细胞里,CB1的激动剂ACEA(Arachidonyl-2'-chloroethylamide)可以上调CB1 mRNA的表达,而分别转染了miR-29a-3p、miR-29b-3p和miR-29c-3p的mimics后可以抑制这一过程;同时对J774A.1细胞转染miR-29a-3p、miR-29b-3p和miR-29c-3p的mimics可以阻碍ACEA诱导的J774A.1细胞的迁移。结论 miR-29s通过下调CB1的表达抑制了ACEA促J774A.1细胞迁移的活性。Objective To investigate whether miR-29a-3p,miR-29b-3p and miR-29c-3p affect cannabinoid receptor 1(CB1)expression and cell migration in J774A.1 cells.Methods RT-qPCR was used to detect CB1 mRNA expression in J774A.1 cells.Boyden chamber assay was used for the detection of cell migration.Results In this study,we presented evidences that CB1 agonist Arachidonyl-2′-chloroethylamide(ACEA)upregulates the expression of CB1 mRNA in J774A.1 cells.Whereas miR-29a-3p or miR-29b-3p or miR-29c-3p mimics blocks the increase of CB1 mRNA level and ACEA-induced cell migration in J774A.1 cells.Conclusion miR-29s inhibit ACEA-induced J774A.1 cell migration via downregulating CB1 expression.

关 键 词：MIR-29 大麻素受体1 细胞迁移 

分 类 号：Q2[生物学—细胞生物学]