不同温度下凡纳滨对虾和中国明对虾对白斑综合征病毒(WSSV)耐受性比较  

The Difference of Tolerance to White Spot Syndrome Virus Between Litopenaeus vannamei and Fenneropenaeus chinensis at Different Temperatures

作　　者：冯亚萍[1,2,3] 孔杰 罗坤[1,2] 栾生 李旭鹏[1,2] 史晓丽 曹家旺[1,2,3] 王明珠 王军[1,2,3] 董丽君 孟宪红[1,2] FENG Yaping;KONG Jie;LUO Kun;LUAN Sheng;LI Xupeng;SHI Xiaoli;CAO Jiawang;WANG Mingzhu;WANG Jun;DONG Lijun;MENG Xianhong(Key Laboratory of Sustainable Development of Marine Fisheries,Ministry of Agriculture,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071;Laboratory for Marine Fisheries Science and Food Production Processes,Qingdao National Laboratory for Marine Science and Technology,Qingdao 266071;College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306)

机构地区：[1]农业部海洋渔业可持续发展重点实验室,中国水产科学研究院黄海水产研究所,青岛266071 [2]青岛海洋科学与技术国家实验室,海洋渔业科学与食物产出过程功能实验室,青岛266071 [3]上海海洋大学水产与生命学院,上海201306

出　　处：《渔业科学进展》2018年第2期120-127,共8页Progress in Fishery Sciences

基　　金：中国水产科学研究院黄海水产研究所基本科研业务费(20603022017001)、国家自然科学基金(41676148)、泰山学者良种工程项目和现代农业产业技术体系专项资金(CARS-48)共同资助

摘　　要：本研究分别对凡纳滨对虾"壬海1号"(Litopenaeus vannamei"Renhai No.1")和中国明对虾"黄海2号"(Fenneropenaeus chinensis"Huanghai No.2")在3种温度条件下(24℃、28℃和32℃),采用单尾定量口饲感染白斑综合征病毒(White spot syndrome virus,WSSV)的方法进行感染实验,比较2种对虾在不同温度情况下对WSSV的耐受性差异(L代表凡纳滨对虾,F代表中国明对虾)。结果显示,L-24℃和F-24℃组的平均存活时间分为(184.05±69.56)h和(101.68±38.45)h;L-28℃和F-28℃组的平均存活时间分别为(100.25±26.79)h和(73.38±22.22)h,相同温度组内均存在显著性差异(P<0.05);截至第15天,L-32℃和F-32℃组的存活率分别为45.74%和23.47%。3个温度组对虾在50%的死亡率时的存活时间分别为178 h和98 h、98 h和74 h、292 h和78 h;死亡高峰时间分别为第5天和第4天、第5天和第4天、第10天和第4天。另外,分别在感染后的12 h、1 d、2 d、3 d、4 d、5 d、6 d、7 d、15 d共9个时间点对每组对虾进行活体取样,利用实时荧光定量RT-PCR技术对其进行病毒载量检测,从对虾体内肌肉组织病毒载量的角度探寻不同对虾抗病性能的差异。6 d时,L-24℃和F-24℃组对虾肌肉内病毒载量分别达到(2.97×10~6±7.44×10~6)和(8.08×10~6±3.22×10~6)copies/ng DNA,差异极显著(P<0.01),L-28℃和F-28℃组分别达到(6.73×10~6±1.49×10~6)和(1.20×10~7±6.15×10~5)copies/ng DNA,差异极显著(P<0.01);15 d,L-32℃和F-32℃组分别达到(5.18×10~4±4.32×10~4)和(3.78×10~4±8.97×10~4)copies/ng DNA,差异显著(P<0.05)。研究表明,2种对虾在3种温度环境下感染WSSV后,凡纳滨对虾耐受WSSV能力要高于中国明对虾;不同温度下同种对虾肌肉体内WSSV的增殖能力从强到弱依次为28℃组、24℃组和32℃组。The present study evaluated the effects of white spot syndrome virus(WSSV)infection in Litopenaeus vannamei‘Renhai No.1’and Fenneropenaeus chinensis‘Huanghai No.2’at various temperatures(24℃,28℃,and 32℃)using quantitative feeding method(L represents L.vannamei,and F represents F.chinensis).The results showed that the average survival time of group L-24℃,F-24℃,L-28℃,and F-28℃was(184.05±69.56)h,(101.68±38.45)h,(100.25±26.79)h,and(73.38±22.22)h,respectively.The survival time between two kinds of shrimps at same temperature was significantly different(P<0.05).At the 15th d,the survival rate of group L-32℃and F-32℃was 45.74%and 23.47%,respectively.The time of 50%mortality rate of two shrimps at three temperatures was 178 h and 98 h,98 h and 74 h,292 h and 78 h,respectively.The time of death peak of two shrimps among three temperatures was 5 d and 4 d,5 d and 4 d,10 d and 4 d,respectively.The virus loads in muscle of L-24℃and F-24℃at 144 h was(2.97×106±7.44×106)and(8.08×106±3.22×106)copies/ng DNA with the highly significant difference(P<0.01).The virus loads in muscle of L-28℃and F-28℃at 144 h was(6.73×106±1.49×106)and(1.20×107±6.15×105)copies/ng DNA with highly significant difference(P<0.01).The virus loads of muscle of L-32℃and F-32℃at the 15th d was(5.18×103±4.32×103)and(3.78×104±8.97×103)copies/ng DNA with a significant difference(P<0.05).These results indicates that L.vannamei are more tolerant to WSSV infection than F.chinensis,and the ability of multiplication of WSSV in muscle at different temperatures was 28℃>24℃>32℃.

关 键 词：凡纳滨对虾 中国明对虾 不同温度 白斑综合征病毒(WSSV) 耐受性 

分 类 号：S917.4[农业科学—水产科学]