纳米银抗菌凝胶对大鼠深Ⅱ度烫伤创面愈合及巨噬细胞数量和微血管密度的影响  

Effect of Nano Silver Antibacterial Gel on the Deep Ⅱ Degrees of Rat Scalded Wound Healing and the Number of Macrophages, the Microvascular Density

作　　者：张紫豪 高素玥 崔文浩 ZHANG Zi-hao;GAO Su-yue;CUI Wen-hao(Chinese PLA General Hospital,Beijing 100000,China;the Second Hospital Affiliated to Suzhou University,Suzhou 215004,Jiangsu,China;the First Peopled Hospital of Jinzhong,Jinzhong 030600,Shanxi,China)

机构地区：[1]中国人民解放军总医院,北京100000 [2]苏州大学附属第二医院,江苏苏州215004 [3]晋中市第一人民医院,山西晋中030600

出　　处：《中国美容医学》2018年第3期104-107,共4页Chinese Journal of Aesthetic Medicine

摘　　要：目的:观察纳米银抗菌凝胶联合重组人粒细胞巨噬细胞集落刺激因子(rhGM-CSF)对大鼠深Ⅱ度烫伤创面愈合、巨噬细胞数量以及微血管再生的影响。方法:选取健康SD大鼠114只(6只作为非烫伤正常对照组,不作任何处理),剩余SD大鼠按照随机数字法分为3组,rhGM-CSF组、联合组和模型组,每组36只。制作深Ⅱ度烫伤创面模型,rhGM-CSF组大鼠创面涂抹rhGM-CSF,联合组应用纳米银抗菌凝胶和rhGM-CSF涂抹,模型组不作任何处理。比较各组创面愈合率,创面巨噬细胞数量以及微血管密度数量。结果:同一时间点,rhGM-CSF组和联合组愈合率均高于模型组,且联合组愈合率显著大于rhGM-CSF组,差异有统计学意义(P<0.05);联合组第3、11、14天创面浸润巨噬细胞数量均少于模型组和rhGM-CSF组(P<0.05);第3、7、11天,联合组微血管数量显著高于模型组和rhGM-CSF组,差异有统计学意义(P<0.05)。结论:纳米银抗菌凝胶联合rhGM-CSF治疗深Ⅱ度烫伤效果确切,可以通过影响烫伤创面巨噬细胞数量和微血管生成促进烫伤创面愈合。Objective To observe the effect of nano silver antibacterial gel joint rhGM-CSF on the deep II degrees of rat scalded wound healing and the number of macrophages,the microvascular density.Methods A total of 114 healthy SD rats(6 rats served as non scald normal control group,without any treatment)were selected,the remaining SD rats were randomly divided into 3 groups,the rhGM-CSF group,the combination group and the model group,36 rats in each group.The deep second degree scald wound model was made,the rhGM-CSF group was treated with rhGM-CSF,the combination group was treated with nano silver antibacterial gel and rhGM-CSF,and no treatment was given to rats of the model group.The rate of wound healing,the number of macrophages and the number of microvessel density in each group were compared.Results At the same time,the healing rate of the rhGM-CSF group and the combination group were higher than those of the model group,and the combination group was significantly higher than the rhGM-CSF group,the difference were statistically significant(P<0.05),The number of macrophages in the combination group on the 3,11,14d were less than those of the model group and the rhGM-CSF group(P<0.05).The number of microvessels in the combination group on the 3,7,lid were significantly higher than those of the model group and the rhGM-CSF group(P<0.05).Conclusion The effect of nano silver antibacterial gel joint rhGM-CSF treatment was exact,and can promote the healing of scalded wounds by affecting the number and angiogenesis of bum wounds.

关 键 词：纳米银抗菌凝胶 重组人粒细胞巨噬细胞集落刺激因子 烫伤 巨噬细胞 微血管 

分 类 号：R644[医药卫生—外科学]