丹参酮生物合成分子调控机制的研究进展  

Research progress on molecular regulation mechanism involved in the biosynthetic pathway of tanshinone in Salvia miltiorrhiza

作　　者：李珅 林爱真 杨媛 沈亚芳 饶盈 羊健[4] 刘云辉 王洋 周伟 LI Shen;LIN Aizhen;YANG Yuan;SHEN Yafang;RAO Ying;YANG Jian;LIU Yunhui;WANG Yang;ZHOU Wei(The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province,School of Agriculture and Food Science,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China;The Agricultural Public Service Center in Xiyang Town,Taishun 325509,Zhejiang,China;Forest Farm of Tianmu Mount,Hangzhou 311311,Zhejiang,China;Key Labortary of Biotechnology in Plant Protection of Ministry of Agriculture of the People’s Republie of China and Zhejiang Province,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,Zhejiang,China)

机构地区：[1]浙江农林大学农业与食品科学学院浙江省农产品品质改良技术研究重点实验室,浙江杭州311300 [2]浙江省泰顺县西旸镇农业公共服务中心,浙江泰顺325509 [3]临安天目山林场,浙江杭州311311 [4]浙江省农业科学院农业部/浙江省植保生物技术重点实验室,浙江杭州310021

出　　处：《浙江农林大学学报》2018年第2期367-375,共9页Journal of Zhejiang A&F University

基　　金：农业部/浙江省植保生物技术重点实验室开放基金资助项目;国家自然科学基金青年基金资助项目(31501394);浙江农林大学大学生科研训练项目(113-2013200006)

摘　　要：丹参Salvia miltiorrhiza是中国的一种传统中草药,对心脑血管疾病的预防和治疗效果显著。由于丹参药用原材料需求量大,其品质越来越受到重视。丹参酮为丹参主要药效成分之一,如何利用最新研究成果进行创新以提高丹参药材的品质是当下研究的热点。论文围绕丹参酮生物合成相关基因和转录调控因子的克隆与功能研究、诱导子促进丹参酮代谢合成的分子机制等方面最新研究展开综述;同时总结了以往研究中存在的问题,并结合自身研究对研究前景进行分析后指出:为了从分子水平上全面阐明丹参酮生物合成的分子调控机制,后续研究应首先围绕丹参酮代谢合成关键调控因子可变剪接的挖掘与分子机制解析;其次是非编码核糖核酸(RNA)在丹参酮代谢合成过程中的调控作用研究;最后是丹参酮合成生物学的探索性研究。Salvia miltiorrhiza,a traditional Chinese herbal medicine,is widely used in preventing and treating cardiovascular diseases.Due to the increasing demand of raw materials of S.miltiorrhiza,its quality has attracted more and more attentions.Tanshinone is one of the main pharmacodynamical components.How to make innovations to improve the content of effective pharmacodynamical component and improve the quality of S.miltiorrhiza based on the existing research is an important work for the researchers.In this study,the latest research progresses on the cloning and identification of genes and transcription factors involved in the biosynthesis pathway of tanshinones,the molecular mechanism of how the elicitors to improve the biosynthesis of tanshinones were reviewed.And then the problems of existing research and the future research direction involved in our recent research were also sorted out and discussed.In order to get a comprehensive knowledge about the molecular mechanism on the production of tanshinones,researchers should pay more attention to three key items.Firstly,alternative splicing of key regulatory factors underlying the biosynthesis of tanshinones should be discovered and identified.Secondly,regulation of the biosynthesis of tanshinones medidated by non-coding RNA shoud be paid more attention.Finally,we should pay more efforts to realize the fact of production of tanshinones using the stratagies of synthetic biology.

关 键 词：植物学 丹参 丹参酮 合成途径 分子机制 综述 

分 类 号：S718.3[农业科学—林学]