人参蛋白质不同提取方法及含量的比较  

Comparision on Different Extraction Methods and Contents of Panax ginseng Protein

作　　者：任雨贺 刘淑莹 万茜淋[1] REN Yu-he;LIU Shu-ying;WAN Xi-lin(Changchun University of Chinese Medicine,Jilin Ginseng Academy,Changchun,Jilin 130117;Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun,Jilin 130022)

机构地区：[1]长春中医药大学,吉林省人参科学研究院,吉林长春130117 [2]中国科学院长春应用化学研究所,吉林长春130022

出　　处：《安徽农业科学》2018年第11期1-3,9,共4页Journal of Anhui Agricultural Sciences

基　　金：吉林省中医药科技项目(2017015);吉林省科技发展计划项目(201603003YY);国家自然科学基金面上项目(21475012)

摘　　要：[目的]优选人参蛋白质提取的最佳方法,并比较不同生长年份对人参蛋白质含量的影响。[方法]比较丙酮沉淀法、聚乙二醇6000沉淀法、聚乙二醇20000沉淀法、酚提取法、Trizol提取法5种人参蛋白质的提取方法,及不同生长年份的人参蛋白质,采用Bradford方法测定蛋白质含量,SDS-PAGE电泳鉴定蛋白。[结果]丙酮提取法得到的蛋白质纯度最高,电泳条带最清晰完整,且人参蛋白质的含量随生长年份增加而升高。[结论]丙酮沉淀法是一种较好的适用于人参蛋白质提取的方法,5年生人参蛋白质含量最高。[Objective]The best method of extraction of ginseng protein was optimized,and the effect of different growth years on protein content was compared.[Method]The extraction method of five kinds of ginseng protein was studied by acetone precipitation method,polyethylene glycol 6000 precipitation method,polyethylene glycol 20000 precipitation method,phenol extraction method and Trizol extraction method,and different growth years of ginseng protein.The protein content was determined by Bradford method,SDS-PAGE identification of proteins.[Result]The purity of the protein was the highest in the acetone extraction method,and the electrophoretic bands were the most clear and complete,and the content of ginseng protein increased with the growth year.[Conclusion]Acetone precipitation method was a better method for the extraction of ginseng proteins.The protein content of ginseng was the highest in 5 years.

关 键 词：人参蛋白 提取方法 SDS-PAGE电泳 

分 类 号：S-3[农业科学]