北京市昌平区2016年健康人群脑膜炎奈瑟菌携带状况、血清群及药物敏感性  

Analysis of presence status,serotype distribution and antibiotic susceptibility of Neisseria meningitidis among healthy residents in Changping district of Beijing,2016

作　　者：牛桓彩[1] 吴杨[1] 舒高林 高进玺[1] NIU Huan-cai;WU Yang;SHU Gao-lin;GAO Jin-xi(Changping District Center for Disease Control and Prevention,Beijing 102200,China)

机构地区：[1]北京市昌平区疾病预防控制中心微生物检验科,北京102200

出　　处：《江苏预防医学》2018年第2期131-134,共4页Jiangsu Journal of Preventive Medicine

摘　　要：目的了解2016年北京市昌平区健康人群中脑膜炎奈瑟菌的携带率和血清群分布,以及对抗生素的敏感性。方法参照《流行性脑脑膜炎奈瑟菌诊断标准》WS 295—2008进行菌株分离及鉴定,用PCR法对菌株进行血清分群,用K-B纸片法对菌株进行抗生素药物敏感性实验,对分离的其他口腔细菌进行16SrRNA测序鉴定。结果收集2016年昌平区2016年9—10月健康人群咽拭子共计252份,分离得到11株(4.37%)脑膜炎奈瑟菌(Nm):属于4个血清群,依次为B群(6株),W135群(3株),C群(1株),X群(1株);抗生素药敏试验结果表明,对头孢噻肟,头孢曲松,美罗培南,利福平完全敏感(100.00%),对环丙沙星,复方新诺明完全耐药(100.00%);27.27%的菌株对四环素耐药,54.55%的菌株对头孢西丁耐药,36.36%的菌株对阿奇霉素不敏感。结论昌平区健康人群携带脑膜炎奈瑟菌以B群为主,对头孢类,碳青霉烯类,安莎霉素类药物敏感,对氟喹诺酮类,磺胺类耐药,并出现对大环内酯类药物不敏感菌株。Objective To study the presence status,serotype distribution and antibiotic susceptibility of Neisseria meningitides(N.meningitidis)among healthy residents in Changping district of Beijing,2016.Methods The N.meningitidis strains were isolated and identified according to the WS 295-2008 Epidemic cerebrospinal meningitis diagnosis standard.The serotype was determined by PCR methods.The antibiotic susceptibility was tested by K-B disk diffusion method.The other isolated strains from oral cavity were identified by 16S rRNA sequencing.Results A total of 11 N.meningitidis strains were isolated from 252 throat swab specimens collected among healthy residents in Changping district from Sep to Oct in 2016,belonging to 4 serotypes:serotype B(n=6),serotype W135(n=3),serotype C(n=1)and serotype X(n=1).All isolates were sensitive to Cefotaxime,Ceftriaxone,Meropenem,Rifampicin,and were resistant to Ciprofloxacin and Ttrimethoprim/Sulfamethoxazole.The resistant rates to tetracycline,cefoxitin were 27.27%and 54.55%,respectively.36.36%were not susceptible to azithromycin.Conclusion The Serotype B strains were the dominant serotype of N.meningitidis among healthy residents in Changping district.The isolated strains were sensitivity to cephalosporins,carbapenems,ansamycins and resistant to quinolones and sulfonamides.Some strains were not sensitive to macrolides.

关 键 词：脑膜炎奈瑟菌 血清群分布 药物敏感性 

分 类 号：R117[医药卫生—公共卫生与预防医学]