低强度脉冲超声对兔膝骨关节炎软骨细胞外基质修复的影响及作用机制  

Effect and mechanism of low-intensity pulsed ultrasound on the repair of chondrocyte extracellular matrix in rabbits with knee osteoarthritis

作　　者：刘伟[1] 杨惠咏 薛海英[1] 张志明[1] 徐家友[1] LIU Wei;YANG Hui-yong;XUE Hai-ying;ZHANG Zhi-ming;XU Jia-you(The First People's Hospital in Shangqiu City,Henan Shanqiu 476100,China)

机构地区：[1]商丘市第一人民医院,河南商丘476100

出　　处：《江苏预防医学》2018年第2期162-164,共3页Jiangsu Journal of Preventive Medicine

基　　金：河南省自然科学基金(2011CDB512)

摘　　要：目的探讨低强度脉冲超声对兔膝骨关节炎软骨细胞外基质修复的影响及其作用机制,为临床膝骨关节炎治疗方案的制定提供理论依据。方法选取90只成年雌性家兔,随机分为实验组和对照组,各45只。通过Hulth法建立膝骨关节炎模型,2周后实验组接受低强度脉冲超声治疗,对照组接受假低强度脉冲超声治疗。在治疗第2、4、8周两组各随机处死15只家兔,对比两组家兔的改良Mankin评分以及基质金属蛋白酶(MMPs)、蛋白多糖Ⅱ型胶原的检测结果,蛋白多糖和Ⅱ型胶原的相对表达量和关节软骨中一氧化氮浓度的变化情况。结果第2、4、8周,观察组的改良Mankin评分以及MMP-3、MMP-7、MMP-13的阳性细胞胞浆平均光密度值均低于对照组,蛋白多糖和Ⅱ型胶原的阳性细胞胞浆平均光密度值均高于对照组(P值均<0.05);观察组关节软骨中一氧化氮浓度以及MMP-3、MMP-7、MMP-13的相对表达量均显著低于对照组,蛋白多糖和Ⅱ型胶原的相对表达量均显著高于对照组(P值均<0.05)。结论低强度脉冲超声可抑制兔膝骨关节炎关节软骨MMPs的表达,降低一氧化氮的含量,减少软骨细胞外基质的降解,有利于关节软骨损伤的修复。Objective To investigate the influence and mechanism of low-intensity pulsed ultrasound on the repair of chondrocyte extracellular matrix in rabbits with knee osteoarthritis;to provide theoretical basis for formulating clinical treatment of knee osteoarthritis.Methods A total of 90 adult female rabbits were randomly divided into experimental group and control group,with 45 rabbits in each group.Knee osteoarthritis model was established with Hulth method.Two weeks later,rabbits in the experimental group received low-intensity pulsed ultrasound treatment,and rabbits in the control group were given sham low-intensity pulsed ultrasound treatment.Fifteen rabbits in each group were randomly sacrificed at 2,4,8 weeks of treatment.Modified Mankin score results,levels of matrix metalloproteinase(MMPs),proteoglycan and type II collagen,proteoglycan and type II collagen,as well as nitric oxide concentration in articular cartilage were compared between control group and experimental group.Results The modified Mankin scores,the mean optical density of MMP-3,MMP-7,MMP-13-positive cell cytoplasm in the experimental group were significantly lower than those in the control group,while the mean optical density of proteoglycan and type II collagen-positive cytoplasm were significantly higher than those in the control group at 2,4,8 weeks(all P<0.05).The nitric oxide concentration in articular cartilage and relative expression of MMP-3,MMP-7,MMP-13 in the experimental group were significantly lower than that in the control group,while the relative expression levels of proteoglycan and type II collagen was significantly higher than those of control group(all P<0.05).Conclusion The low-intensity pulsed ultrasound can inhibit MMPs expression in articular cartilage,reduce nitric oxide content,decrease extracellular matrix degradation,and promote articular cartilage repair after injury of rabbits.

关 键 词：低强度脉冲超声 兔膝骨关节炎 软骨细胞 基质金属蛋白酶 蛋白多糖 Ⅱ型胶原 基质修复 作用机制 

分 类 号：R684[医药卫生—骨科学]