(131)~I标记卵清蛋白及其载(131)~I-OVA的PLGA/DDAB纳米粒SPECT显像  

Radiolabeling Ovalbumin with (131)~I and (131)~I-OVA-PLGA/DDAB Nanoparticles to Vivo SPECT Imaging

作　　者：马文颜 康磊[2] 王连艳[3] 霍焱[2] 申镐源[2] 王艳萍[1] MA Wen-yan;KANG Lei;WANG Lian-yan;HUO Yan;SHEN Hao-yuan;WANG Yan-ping(College of Food Engineering and Biotechnology,Tianjin University of Science&Technology,Tianjin 300457,China;Peking University First Hospital,Beijing 100034,China;State Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Chinese Academy of Science,Beijing 100190,China)

机构地区：[1]天津科技大学食品工程与生物技术学院,天津300457 [2]北京大学第一医院,北京100034 [3]中国科学院过程工程研究所生化工程国家重点实验室,北京100190

出　　处：《科学技术与工程》2018年第8期27-33,共7页Science Technology and Engineering

基　　金：国家科技重大专项子课题(2016ZX10004001-005)资助

摘　　要：制备载(131)~I-OVA的荷正电聚乳酸-乙醇酸(PLGA/DDAB)纳米粒,探讨其在活体动物体内的生物分布与转运研究。氯胺T法对卵清蛋白(OVA)进行(131)~I标记,Sephadex G 25纯化标记抗原,纸层析法测定标记物的生物活性和体外稳定性。纳米沉淀法制备PLGA/DDAB纳米球,将其与(131)~I-OVA共混吸附,制备载(131)~I-OVA的PLGA/DDAB纳米粒。动物试验选择C57/BL6小鼠,肌肉注射载(131)~I-OVA的PLGA/DDAB纳米粒,分析(131)~I-OVA在小鼠组织器官中的分布情况,并于注射疫苗制剂后不同时间行SPECT正位静态显像,观察小鼠不同组织内放射性浓聚情况。结果显示,标记抗原经纯化后,测得(131)~I-OVA比活度达32~42μCi·μg-1,标记率达(71.92±0.08)%,放射性化学纯度为(85.94±0.15)%。标记物在新鲜血清中存放72 h后,仍保持很高的反应活性,表明标记的OVA稳定性良好。采用纳米沉淀法制备PLGA/DDAB纳米球,平均粒径、分散系数和Zeta电位分别为122.8 nm、0.084和+31.5 m V。将其与(131)~I-OVA共混吸附,抗原携载率达(89.60±0.21)%。注射载(131)~I-OVA的PLGA/DDAB纳米球疫苗制剂,与无佐剂疫苗相比,抗原在注射部位消失的速度更为缓慢。结果表明,成功制备了载(131)~I-OVA的PLGA/DDAB纳米球,生物活性较好,可用于抗原在活体动物的生物分布与转运研究。To prepare the PLGA/DDAB nanoparticles containing 131 I-labeled OVA,and to analyze its bio-distribution and transport in vivo,the ovalbumin(OVA)was labeled with the chloramine T method,labeled OVA was purified by Sephadex G 25.The PLGA/DDAB nanoparticles were prepared by nano-precipitation method,and 131 I-OVA was loaded on nanoparticles by adsorption.C57/BL6 mice were selected as experiment animals.The distribution of antigens in different organs was measured at the indicated time points after intramuscular injection.Anteroposterior static imaging was performed with SPECT after injection to observe the radioactivity in organs.The specific activity,the labeling rate and the chemical purity of 131 I-OVA were 32~42μCi·μg-1,(71.92±0.08)%and(85.94±0.15)%,respectively.The marker remained high reactivity in the fresh serum for 72 h,the good reactivity of labeled OVA was indicated.The PLGA/DDAB nanoparticles were prepared by nano-precipitation method.The mean diameter,polydisperisity index,zeta potential were 122.8 nm,0.084 and+31.5 mV.The PLGA/DDAB nanoparticles and 131 I-OVA were mixed with antigen loading efficiency of 89.6%.After injection of the PLGA/DDAB nanoparticles containing 131 I-OVA,the antigen disappeared slower at the injection site as compared to the no adjuvant vaccine.The PLGA/DDAB nanoparticles containing 131 I-OVA can be successfully prepared and shows a high biological activity,it can be used for the study of biodistribution and biological transport of antigen.

关 键 词：放射性核素131I PLGA 纳米粒子 SPECT显像 

分 类 号：R817.9[医药卫生—影像医学与核医学]