海洛因对腹腔巨噬细胞分泌白细胞介素-1的影响  

Effect of Heroin on the Secretion of Interleukin-1 by Peritoneal Macrophages

作　　者：牛勇 李洋[2] 卢延旭[3] NIU Yong;LI Yang;LU Yanxu(Department of Criminal Investigation,Ministry of Public Security,Beijing 100741,China;Institute of Forensic Science,Ministry of Public Security,Beijing 100038,China;Criminal Investigation Police University of China,Shenyang 110854,China)

机构地区：[1]公安部刑事侦查局,北京100741 [2]公安部物证鉴定中心,北京100038 [3]中国刑事警察学院,沈阳110854

出　　处：《刑事技术》2018年第2期135-137,共3页Forensic Science and Technology

基　　金：中央级公益性科研院所基本科研业务费专项资金项目(No.2016JB041)

摘　　要：目的研究海洛因对腹腔巨噬细胞分泌白细胞介素-1的影响。方法体外培养腹腔巨噬细胞,用胸腺细胞增殖法检测白细胞介素-1的活性。结果海洛因处理后腹腔巨噬细胞分泌白细胞介素-1增强,通过紫外分光计检测λ=405nm处海洛因处理组A值为700±82.82,而空白对照组的A值为535.6±24.66(P<0.05)。结论海洛因可以通过影响白细胞介素-1进一步影响机体的免疫功能。Objective To study the influence of heroin on the peritoneal macrophages to secrete interleukin(IL)-1.Methods Mouse peritoneal macrophages were cultured in vitro with addition of heroin into the routine medium and measured of the IL-1 activity via thymocyte proliferation method.Results After treated with heroin,the mouse peritoneal macrophages secreted IL-1 increasingly.Through measurement by an ultraviolet spectrophotometer at theλ=405nm,an A value was obtained about 700±82.82 of the IL-1 activity in the heroin-treated group compared with 535.6±24.66 in the control group(p<0.05).Conclusion heroin could modulated the mouse immunologic function by promoting IL-1secretion.

关 键 词：海洛因 腹腔巨噬细胞 白细胞介素-1 

分 类 号：DF795.1[医药卫生—法医学]