丝素/海藻酸钠膜韧性的优化及膜释药机理分析  

Optimizing the Toughness of Silk Fibroin/Sodium Alginate Membrane and Analyzing the Drug Release Mechanism from the Membrane

作　　者：高保东[1] 钟红荣 吴婷芳 谭翠 张岩[1] 徐水[1] GAO Baodong;ZHONG Hongrong;WU Tingfang;TAN Cui;ZHANG Yan;XU Shui(College of Biotechnology,Southwest University,Chongqing 400715)

机构地区：[1]西南大学生物技术学院,重庆400715

出　　处：《材料导报》2018年第7期1197-1201,共5页Materials Reports

基　　金：重庆市蚕丝纤维新材料工程技术研究中心专项研究(SILKGCZX009);江苏高校省级重点实验室开放研究课题(KJS1508)

摘　　要：首先在丝素(SF)溶液中添加既定浓度的海藻酸钠(SA)溶液和一定体积的75%甘油、50%戊二醛以及模型药物罗丹明B(4g/L),将均匀混合液以浇铸方法制备丝素/海藻酸钠(SF/SA)膜模型药物缓释敷料;然后利用响应曲面法筛选最佳韧性(以断裂伸长率表示)SF/SA膜的制备参数;最后对优化膜的韧性和药物释放动力学模型进行检验与分析。实验得出,在浓度为2.5%的丝素溶液中,添加等体积0.96%浓度的海藻酸钠溶液,且甘油和戊二醛添加量分别为膜液总体积1.41%和4.47%时达到最优制备条件,此时膜的断裂伸长率为228.36%。检验结果显示相对对照组,优化组的断裂伸长率扩大了18.14倍;根据回归方程计算得出影响断裂伸长率的主效因素是甘油,且其与戊二醛具有显著协同作用。另外,根据优化组的释药结果分析表明:该体系具有药物缓释功效,释药动力学归属于Fickan模型。以上结果有利于丝素/海藻酸钠膜舒适型药物缓释敷料的制备,可为该生物材料的应用研究提供参考。Firstly,sodium alginate(SA)solutions with predetermined concentrations,and certain volume of 75%glycerol,50%glutaraldehyde and model drug rhodamine B(4 g/L),were added into silk fibroin(SF)solution to form a series of homoge-neous mixtures which were used to prepare sustained-releasing silk fibroin/sodium alginate(SF/SA)membranes by casting method.Secondly,the preparation parameters were optimized via the response surface methodology aiming at achieving the highest product toughness(characterized as elongation at break).Lastly,the optimized SF/SA membrane’s toughness and the drug release kinetic model were tested and analyzed.We figured out the optimum preparation conditions for SF/SA membrane as:adding 0.96%(concentration)sodium alginate solution into an equal volume of 2.5%(concentration)silk fibroin solution,and setting the volume fractions of glycerol and glutaraldehyde to the whole mixture at 1.41%and 4.47%,respectively.And these optimized parameters could lead to a SF/SA membrane with an elongation at break of 228.36%,which was 18.14 times higher than that of the control group.The calculation result via regression equation suggested that the main factor affecting the elongation at break was glycerol dosage,and it had a significant synergistic effect with glutaraldehyde dosage.The drug release analysis confirmed the sustained-releasing cha-racter of the optimized group along with a pharmacokinetics coinciding with the Fickan model.The above results are favorable to the preparation of silk fibroin/sodium alginate membrane as the comfort and sustained-releasing dressing,which can provide reference for biomaterials application.

关 键 词：丝素 海藻酸钠 响应曲面法 药物缓释 断裂伸长率 

分 类 号：TQ341.5[化学工程—化纤工业]