橘络活性成分小分子果胶治疗非小细胞肺癌作用及机制研究  

Effect and Mechanism of Low-Molecular-Weight Citrus Pectin on Non-Small Cell Lung Cancer

作　　者：张雯珺[1] 屠凌岚[1] 吴丽梅 高海峰 黄萍萍 郑晓亮[1] 王孝举[1] ZHANG Wen-jun;TU Ling-lan;WU Li-mei;GAO Hai-feng;HUANG Ping-ping;ZHENG Xiao-liang;WANG Xiao-ju(Center for Molecular Medicine,Zhejiang Academy of Medical Sciences,Hangzhou 310013,China;Hangzhou Joyful Biotechnology Co.,Ltd,Hangzhou 310052,China)

机构地区：[1]浙江省医学科学院分子医学中心,杭州310013 [2]杭州络通生物科技有限公司,杭州310052

出　　处：《天然产物研究与开发》2018年第4期575-581,共7页Natural Product Research and Development

基　　金：浙江省自然科学基金(LY13H310004)

摘　　要：本文观察橘络活性成分小分子果胶在离体细胞水平及整体动物模型中对非小细胞肺癌的治疗作用并初步探索其作用机制。小分子果胶处理人非小细胞肺癌PC-9细胞72 h后,MTS法检测细胞活率,流式细胞仪检测细胞凋亡率;以PC-9细胞裸小鼠皮下移植瘤模型观察小分子果胶体内对肿瘤生长的抑制作用,免疫组化法检测肿瘤组织Ki67表达水平,TUNEL法检测组织中肿瘤细胞凋亡程度。结果表明小分子果胶(1.5~3 mg/m L)可显著抑制PC-9细胞增殖,与对照组相比细胞凋亡率显著升高,且具有浓度依赖性;小分子果胶(56.25 mg/kg)可显著抑制非小细胞肺癌小鼠体内肿瘤生长,肿瘤组织中细胞凋亡率显著升高。研究证明小分子果胶可显著抑制非小细胞肺癌小鼠体内肿瘤生长,该作用可能与小分子果胶抑制肿瘤细胞增殖、诱导细胞凋亡有关。To observe the therapeutic effect of low-molecular-weight citrus pectin(LCP)on non-small cell lung cancer(NSCLC)and to explore its mechanism in vitro and in vivo.Viability of NSCLC PC-9 cell treated with LCP for 72h was measured by MTS assay,and cell apoptosis rate was determined by flow cytometric analysis.The inhibitory effect of LCP on tumor growth was observed by xenograft model of PC-9 cells.The expression level of Ki67 was detected by immunohistochemistry and the apoptosis of tumor cells was detected by TUNEL assay.LCP(1.5-3 mg/mL)can inhibit the proliferation of PC-9 cells,and the apoptotic rate was significantly higher than that of the control group in vitro.LCP(56.25 mg/kg)can inhibit the tumor growth of NSCLC,and tumor cells apoptosis rate were significantly increased in vivo.LCP can significantly inhibit tumor growth of NSCLC,which may be related to the inhibition of tumor cell proliferation and induction of apoptosis.

关 键 词：小分子果胶 非小细胞肺癌 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]