冬虫夏草定殖菌Aspergillus fumigatus中一个新的聚酮类化合物  

New Polyketide Isolated from Aspergillus fumigatus,A Cordyceps-colonizing Fungus

作　　者：李莎[1] 李小华 邱玲[1] 旷歧轩 文新 于思 郭大乐[1] 邓赟[1] LI Sha;LI Xiao-hua;QIU Ling;KUANG Qi-xuan;WEN Xin;YU Si;GUO Da-le;DENG Yun(Key Laboratory of Standardization of Chinese Herbal Medicine,Ministry of Education,State Key Laboratory Breeding of Systematic Research Development and Utilization of Chinese Medical Resources,Co-founded by Sichuan Province and MOST,Pharmacy Department,Chengdu University of Traditional Chinese Me)

机构地区：[1]成都中医药大学药学院中药材标准化教育部重点实验室,四川省中药资源系统研究与开发利用重点实验室,省部共建国家重点实验室培育基地,成都611137

出　　处：《天然产物研究与开发》2018年第4期588-590,共3页Natural Product Research and Development

基　　金：国家自然科学基金面上项目(81373961);国家自然基金国家基础科学人才培养项目(J13100340);四川省科技厅省青年科技创新研究团队专项(2014TD0007)

摘　　要：从冬虫夏草中分离得到一株定殖菌烟曲霉,对其发酵液的乙酸乙酯萃取部位进行化学成分研究,借助正反相硅胶柱色谱及HPLC等方法进行分离纯化,分离得到一个聚酮类新化合物1,利用多种波谱手段(HRESI-MS,1D NMR,2D NMR)确定其结构为为6-methoxy-3-methyl-1H-isochromen-8-ol。化合物1对H1299(人肺癌细胞)、H460(大细胞肺癌细胞)、HGC-27(人胃癌细胞)、A549(人非小细胞肺癌细胞)和mnk-43(人胃癌高分化细胞)五种细胞株均有弱的细胞毒活性。Aspergillus fumigatus,a Cordyceps-colonizing fungus has been isolated from Cordyceps sinensis and its chemical constituents were investigated.A new compound 1 had been isolated from EtOAc extract of the fermentation broth by silica gel,RP C18 column chromatography and preparative HPLC and its structure was elucidated as 6-methoxy-3-methyl-1H-isochromen-8-ol by various spectroscopic data(HR-ESI-MS,1D NMR,2D NMR).Compound 1 was shown to have weak cytotoxic activity against the H1299,H460,HGC-27,A549 and mnk-43.

关 键 词：烟曲霉 冬虫夏草 6-methoxy-3-methyl-1H-isochromen-8-ol 结构鉴定 细胞毒活性 

分 类 号：R284.1[医药卫生—中药学]