藏药印度獐牙菜提取物红外光谱分析及保肝活性比较研究  

Infrared Spectroscopy Analysis and Hepatoprotective Activity of Swertia chirayita Extracts

作　　者：杨红霞[1] 李岑[1] 毕宏涛[1] 魏立新[1] 杜玉枝[1] YANG Hong-xia;LI Cen;BI Hong-tao;WEI Li-xin;DU Yu-zhi(Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Qinghai key laboratory of Tibetan Medicine Pharmacology and Safety Evaluation;Key Laboratory of Tibetan Medicine Research,Chinese Academy of Sciences,Xining 810008,China)

机构地区：[1]中国科学院西北高原生物研究所,青海省藏药药理学和安全性评价研究重点实验室,中国科学院藏药研究重点实验室,西宁810008

出　　处：《天然产物研究与开发》2018年第4期668-675,共8页Natural Product Research and Development

基　　金：中国科学院重点部署项目(KSZD-EW-Z-004);青海省重点实验室专项(2017-ZJ-Y08)

摘　　要：比较藏药材印度獐牙菜不同溶剂提取物指标成分及提取率较高的两种提取物之间的肝保护作用。采用HPLC和红外光谱分析技术分析不同溶剂提取物的指标成分变化;采用CCl4致小鼠肝损伤模型进行水提物和75%乙醇提取物保肝活性药效学研究。印度獐牙菜不同溶剂提取物的红外光谱图中能很好的对应獐牙菜苦苷1280 cm-1处,芒果苷1496和1464 cm-1处的特征峰。不同溶剂提取物的红外光谱之间及其所含目标成分的特征峰强度之间存在明显的差异。提取率较高的水提物和75%乙醇提取物均具有明显的保肝活性,且在0.5~2.0 g/kg的给药剂量范围内,印度獐牙菜75%乙醇提取物保肝活性呈明显的量效关系。The aim of this study was to compare the ingredient and hepatoprotective activity of different extracts of Swertia chirayita.The method of HPLC and infrared spectroscopy was used to compare the ingredient of S.chirayita extracts.Liver injury model induced by CCl 4 in mice was used to compare the hepatoprotective function of water extract and 75%ethanol extract.The results indicated that the intensity of 1280 cm-1 of swertiamarin,1496 and 1464 cm-1 of mangiferin,had positive correlation with the content change in different extracts.There had obvious difference between IR spectra of different extracts and between intensity of targeted ingredient of different extracts.The pharmacology experiments results prov ed the water extracts and 75%ethanol extracts had clear hepatoprotective function,and during the dose range of 0.5-2.0 g/kg,the hepatoprotective activity of 75%ethanol extracts showed obvious dose-effect relationship.

关 键 词：印度獐牙菜 提取物 红外光谱 保肝活性 

分 类 号：R931.6[医药卫生—生药学]