基于分光光度法的海水原位硅酸盐微芯片分析传感器的研制  

Development of Lab-on-Chip Spectrophotometric Silicate Sensor in situ Analysis of Seawater

作　　者：曹煊[1,2] 张述伟[1,2] 褚东志[1,2] 吴宁[1,2] 马海宽 马然[1,2] CAO Xuan;ZHANG Shu-wei;CHU Dong-zhi;WU Ning;MA Hai-kuan;MA Ran(Institute of Oceanographic Instrument,Shandong Academy of Sciences,Qingdao 266001,China;Shandong Ocean Environment Monitoring Technology Key Laboratory,Qingdao 266001,China)

机构地区：[1]山东省科学院海洋仪器仪表研究所,山东青岛266001 [2]山东省海洋环境监测技术重点实验室,山东青岛266001

出　　处：《光谱学与光谱分析》2018年第3期895-900,共6页Spectroscopy and Spectral Analysis

基　　金：国家重点研发计划项目(2016YFC1400803;2016YFC1400801);国家自然科学基金项目(41206076);山东省资助自然科学基金项目(ZR2014DM012);山东省重点研发计划项目(2015GGX103031)资助

摘　　要：基于酸性条件下硅酸盐与钼酸铵反应生成硅钼黄后被还原成硅钼蓝的原理,借助微流控芯片平台,建立基于连续流动-分光光度法快速测定海水硅酸盐的分析传感器,传感器测定周期约为300s,采用双光程方式拓展测量范围,线性测量范围可达0~400μmol·L^(-1),双光程的检出限分别为45.1nmol·L^(-1)和1.6μmol·L^(-1);当海水盐度大于15时,传感器测量准确度和稳定性基本不受盐度影响,且实际海水加标回收率在98.1%~104.0%之间。2015年11月在胶州湾开展了20个站点海试和同步比对试验,结果显示,该系统具有准确度高、简单、集成度高、水样消耗少、抗干扰能力强等优点,可用于海水原位硅酸盐的分析。A new Microfluidic continuous flow analysis sensor is established for the determination of soluble silicate in seawater based on the reaction of silicate with ammonium molybdate to form a yellow silicomolybdate complex,and further reduction to silicomoIybdenum blue by ascorbic acid.Theanalyzing cycle of the sensor is about 300 s,Double optical absorbance path method is adopted in this sensor toexpand the measuring range,and the linear range of the sensor reaches 0~400 mol·L-1 with limit of detection of each absorbance cell were 45.1 nmol·L-1 and 1.6μmol·L-1 respectively.There are no evident effects on the performance of determination of this sensor when samples’salinity is above 15,and the actual water the recovery rate is between 98.1%~104.0%.In November 2015,this sensor was deployed in a research vessel for a sea trail inJiaozhou Bay involving 20 sites,the data acquired from the sensor were compared with those from sampling then by lab analyzing.The results obtained in situ coincide with those from lab analyzing,and indicating This sensor has the advantages of high accuracy,simple and high integration,water consumption,and strong anti-interference ability,and suitable for in situ silicatemonitoring.

关 键 词：硅酸盐 分光光度法 微流控芯片 传感器 海水 

分 类 号：P715.5[天文地球—海洋科学]