不同存储时间对红细胞血红蛋白再释放的影响  

Effect of different storage time on the re-release hemoglobin from red blood cells

作　　者：李姝 张丽华 袁海清 杨丽丽[1] 宋芳[1] 李旭妍 孙明玥 黄世琪[1] 苏燕[1] LI Shu;ZHANG Lihua;YUAN Haiqing;YANG Lili;SONG Fang;LI Xuyan;SUN Mingyue;HUANG Shiqi;SU Yan(Institute of Blood Conservation,Baotou Medical College,Baotou 014060,China;Center Blood Station of Baotou,Baotou)

机构地区：[1]内蒙古科技大学包头医学院血液保护研究所,内蒙古包头014060 [2]包头市中心血站

出　　处：《包头医学院学报》2018年第4期89-90,93,共3页Journal of Baotou Medical College

基　　金：国家自然科学基金(81160214);内蒙古自然科学基金(2016MS0801);包头市科技计划项目(2015C2016-16);包头医学院博士基金(BSJJ201615);内蒙古自治区卫生和计划生育委员会科研计划项目(201701090)

摘　　要：目的:观察不同存储时间对红细胞(red blood cells,RBCs)血红蛋白(hemoglobin,Hb)再释放的影响。方法:募集健康成人志愿献血者,分别采集200 mL全血,滤白后离心去除血浆,将RBCs重悬于50 mL红细胞保护液(MAP)中,4℃保存。于储存期0 d、7 d、14 d、21 d、28 d、35 d、42 d抽取血样5 mL,分别检测红细胞Hb再释放、RBCs形态、聚集指数、相对溶血率、上清丙二醛(MDA)含量及高铁血红蛋白分数(FMet Hb)。结果:随着存储时间延长,红细胞Hb再释放增多,同时伴随RBCs相对溶血率、MDA含量、FMet Hb、变形RBCs、RBCs聚集指数的逐渐增高。结论:RBCs存储损伤可以导致Hb再释放增多。Objective:To observe the effect of different storage time on the re-release hemoglobin(Hb)from red blood cells(RBCs).Methods:Healthy adults were recruited,and 200 ml of whole blood was collected from each of them.After the white blood cells were filtered,plasma was removed by centrifugation;packed RBCs were resuspended in 50ml MAP RBCs protective solution and stored at 4℃.At shelf life of 0,7,14,21,28,35 and 42 d,5 ml blood sample was taken and then the re-released Hb,morphology of RBCs,RBCs aggregation index,relative hemolysis rate,malondialdehyde(MDA)content and the fraction of methemoglobin(FMetHb)were detected.Results:With the prolongation of storage time,the re-release Hb was increased;at the same time the relative hemolysis rate of RBCs,MDA,FMetHb,deformated RBCs,and RBCs aggregation index also increased correspondingly.Conclusion:RBCs storage lesion might increase the re-released Hb.

关 键 词：红细胞(RBCs) 血液存储 血红蛋白释放试验(HRT) 

分 类 号：R457.1[医药卫生—治疗学]