盐胁迫对扁蓿豆幼苗脯氨酸积累及其代谢关键酶活性的影响  

Effect of salt stress on proline accumulation and the activities of the key enzymes involved in proline metabolism in Medicago ruthenica seedlings

作　　者：钟华[1] 董洁[2] 董宽虎[1] ZHONG Hua;DONG Jie;DONG Kuan-hu(College of Animal Science and Veterinary Medicine,Shanxi Agriculture University,Taigu 030801,China;Beijing Science and Technology Information Institute,Beijing 100044,China)

机构地区：[1]山西农业大学动物科技学院,山西太谷030801 [2]北京市科学技术情报研究所,北京100044

出　　处：《草业学报》2018年第4期189-194,共6页Acta Prataculturae Sinica

基　　金："十一五"国家科技支撑计划课题(2007BAD56B01);山西省科技攻关项目(20120311011-1)资助

摘　　要：以野生扁蓿豆为试验材料,采用盆栽试验方法研究不同盐(NaCl、Na_2CO_3)的不同浓度(0、50、100、200、300、400、500mmol·L^(-1))胁迫对扁蓿豆苗期脯氨酸积累及其代谢途径的关键酶活性的影响。结果表明,在不同盐胁迫下,扁蓿豆脯氨酸(Pro)含量、吡咯啉-5-羧酸合成酶(P5CS)活性、鸟氨酸-δ氨基转移酶(δ-OAT)活性均随盐浓度的升高而逐渐增加,而脯氨酸脱氢酶(ProDH)活性随胁迫浓度的升高而降低。扁蓿豆幼苗主动积累Pro是缓解其NaCl和Na_2CO_3胁迫的原因,并由Glu途径和Orn途径协同作用于Pro积累,但以Orn途径为主。试验中,扁蓿豆所承受的NaCl和Na_2CO_3浓度不宜超过400mmol·L^(-1)。In order to study the effects of salt stress on proline accumulation and the activities of the key enzymes involved in proline metabolism a pot experiment was conducted using wild Medicago ruthenica stressed under different NaCl and Na2CO3 solutions;0,50,100,200,300,400 and 500 mmol·L-1.The result showed that,under salt stress,proline content and P5CS andδ-OAT activity gradually increased while ProDH activity declined with increasing salt concentration.The initial accumulation of proline was a major reason for the reduced damage from NaCl and Na2CO3 stress in M.ruthenica seedlings.The Glu and Orn pathways were synergistic to the accumulation of proline,with the Orn pathway playing a leading role.This study suggests that the concentration of NaCl and Na2CO3 should not exceed 400 mmol·L-1 for M.ruthenica.

关 键 词：扁蓿豆 盐胁迫 脯氨酸 吡咯啉-5-羧酸合成酶 鸟氨酸-δ氨基转移酶 脯氨酸脱氢酶 

分 类 号：S541.9[农业科学—作物学]