TUNEL法检测自拟复方葛根片对老龄大鼠子宫的保护作用  

Measuring the protective effect of self-developed Compound Radix Puerariae tablet on uterus in aged rats by using TUNEL

作　　者：陶利[1] 王飞飞 李云江 张淑瑜[1] 毕云生[1] 于燕莉[1] 徐建江[1] TAO Li;WANG Fei-fei;LI Yun-jiang(Department of Pharmacy,the General Hospital of Jinan Military Region,Jinan,Shandong 250031,China)

机构地区：[1]济南军区总医院,山东济南250031 [2]山东中医药大学,山东济南250355

出　　处：《实用医药杂志》2018年第4期340-342,共3页Practical Journal of Medicine & Pharmacy

摘　　要：目的研究自拟复方葛根片对老龄大鼠子宫的保护作用。方法 40只Wistar大鼠随机分成5组(正常组、模型组、复方葛根片低、中、高剂量组),每组8只。按相关试剂盒操作分别测定血清中雌二醇(E2)、促卵泡激素(FSH)、促黄体生成素(LH)、泌乳素(PRL)浓度;采用TUNEL法检测衰老大鼠子宫的细胞凋亡情况。结果与正常组比较,模型组大鼠E2浓度降低,FSH、LH、PRL浓度升高(P<0.01),造模成功;与模型组比较,复方葛根片各用药组中E2浓度升高,FSH、LH、PRL浓度降低(P<0.05或P<0.01)。与正常组比较,模型组大鼠子宫切片中红色荧光明显增多,凋亡指数显著增加(P<0.01);与模型组比较,复方葛根片各用药组中红色荧光明显减少,凋亡指数显著减小(P<0.05或P<0.01)。结论复方葛根片能调节雌激素水平,同时抑制老龄大鼠子宫细胞凋亡,减少凋亡细胞的产生,起到保护子宫,延缓衰老作用。Objective To study the protective effect of Compound Radix Puerariae tablet on the uterus of senile rats.Methods Forty Wistar rats were randomly divided into 5 groups(normal group,model group and low,medium,and high TLC dose group),8 rats in each group.According to the related kit serum estradiol(E2),follicle stimulating hormone(FSH)and luteinizing hormone(LH),prolactin(PRL)concentration were determined;by using TUNEL uterine was detected in cell apoptosis.Results Compared with the normal group,the rats in the model group E2 concentration decreased,FSH,LH,PRL concentration increased(P<0.01),showing the model was successful;compared with the model group,the treatment group of Compound Pueraria tablets in the higher concentration of E2,FSH,LH,PRL decreased(P<0.05 or P<0.01).Compared with the normal group,the red fluorescence of uterine sections in model group were significantly increased,the apoptotic index was significantly increased(P<0.01);compared with the model group,Compound Pueraria tablets and red fluorescence in the high dose group and low dose group were significantly reduced,the rightness decreased,apoptosis index decreased significantly(P<0.05 or P<0.01).Conclusion Compound Radix Puerariae tablet can inhibit the apoptosis of uterus cells,reduce the production of apoptotic cells,regulate the estrogen level,and protect the uterus and has anti-aging effect in the aged model rats.

关 键 词：复方葛根片 衰老模型 细胞凋亡 TUNEL法 雌激素 子宫 

分 类 号：R339.38[医药卫生—人体生理学] R339.22[医药卫生—基础医学]