黄葵胶囊对慢性肾小球肾炎模型大鼠肾组织病理和免疫功能的影响  

Influence of Huangkui Capsule on Renal Pathology and Immune Function in Chronic Glomerulonephritis Rats

作　　者：徐赛华 胡静娜 XU Saihua;HU Jingna(Dispensary of Traditional Chinese Medicine,Yinzhou No.2 Hospital,Ningbo(315100),China)

机构地区：[1]浙江省宁波市鄞州第二医院中药房,宁波315100

出　　处：《浙江中西医结合杂志》2018年第4期274-277,共4页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

基　　金：浙江省宁波市自然科学基金项目(No.2012A610199)

摘　　要：目的观察黄葵胶囊对慢性肾小球肾炎模型大鼠肾组织病理和免疫功能的影响。方法SD大鼠50只随机分为空白对照组、模型组、黄葵胶囊低、中、高剂量组,每组10只,采用小牛血清白蛋白法建立慢性肾小球肾炎大鼠模型,空白对照组和模型组给予生理盐水灌胃,黄葵胶囊低、中、高剂量组分别给予0.5g/kg、1.0g/kg和2.0g/kg的黄葵胶囊灌胃,共8周。观察各组大鼠肾组织病理学变化,检测外周血T淋巴细胞亚群比例和血清肌酐(Scr)、尿素氮(BUN)、尿酸(UA)及肾组织白介素-6(IL-6)、细胞间黏附因子-1(ICAM-1)、转化生长因子-β1(TGF-β1)水平。结果空白对照组大鼠肾小球、系膜基质未见明显病理变化,模型组大鼠肾小球硬化、系膜基质增生,黄葵胶囊各剂量组大鼠肾脏病理变化较模型组明显减轻。模型组大鼠外周血CD3、CD4和CD4/CD8均明显低于空白对照组和黄葵胶囊低、中、高剂量组,差异均有统计学意义[CD3:(41.51±3.84)%比(58.23±4.52)%、(46.31±4.14)%、(50.25±4.37)%、(56.04±4.42)%,P<0.05;CD4:(21.73±2.86)%比(37.61±3.47)%、(26.59±2.97)%、(32.44±3.11)%、(35.31±3.32)%,P<0.05;CD4/CD8:(0.64±1.16)比(1.75±1.68)、(0.86±1.31)、(1.23±1.52)、(1.52±1.54),P<0.05];CD8高于空白对照组和黄葵胶囊低、中、高剂量组,差异均有统计学意义[(33.74±2.46)%比(21.49±2.07)%、(30.81±2.27)%、(26.33±2.05)%、(23.17±2.15)%,P<0.05]。模型组大鼠血清Scr、BUN和UA含量高于空白对照组、黄葵胶囊低、中、高剂量组,差异均有统计学意义[Scr:(68.62±5.74)μmol/L比(45.21±6.72)μmol/L、(62.17±7.23)μmol/L、(58.06±6.18)μmol/L、(52.45±6.85)μmol/L,P<0.05;BUN:(17.29±2.43)mmol/L比(7.26±2.01)mmol/L、(14.67±2.28)mmol/L、(11.05±2.17)mmol/L、(9.14±2.37)mmol/L,P<0.05;UA:(114.37±9.27)μmol/L比(65.68±7.12)μmol/L、(87.53±8.76)μmol/L、(80.49±8.49)μmol/L、(72.66±8.27)μmol/L,P<0.05]。模型组大鼠肾组织IL-6、ICAM-1和TGF-β1水平均高于�Objective To study the influence of Huangkui capsule on inflammatory factor and immune function in chronic glomerulonephritis rats.Methods The chronic glomerulonephritis rats model were established by calf serum albumin method.Forty model rats were randomly divided into model group,Huangkui capsule low-,medium-and high-dose groups,with each group of 10 rats,and other 10 normal rats were set as control group.The control group and model group were given normal saline,and the experimental groups were given 0.5g/kg,1.0g/kg and 2.0g/kgHuangkui capsule,respectively.After 8 weeks,the renal histopathological changes,content of serum creatinine(Scr),blood urea nitrogen(BUN)and uric acid(UA),levels of interleukin-6(IL-6),intercellular adhesion factor-1(ICAM-1)and transforming growth factor-beta 1(TGF-β1),and the ratio of peripheral blood T lymphocyte subsets were compared.Results There was no obvious pathological changes of glomerular and mesangial matrix in the control group,mesangial matrix was proliferated and glomerular sclerosis occurred in the model group,but these kidney pathological changes were significantly reduced in the experimental group.The ratios of peripheral blood T lymphocyte subsets CD3,CD4 and CD4/CD8 in the model group were significantly lower than those in the control group and experimental groups(CD3:41.51%±3.84%vs 58.23%±4.52%,46.31%±4.14%,50.25%±4.37%,56.04%±4.42%,P<0.05;CD4:21.73%±2.86%vs 37.61%±3.47%,26.59%±2.97%,32.44%±3.11%,35.31%±3.32%,P<0.05;CD4/CD8:0.64±1.16 vs 1.75±1.68,0.86±1.31,1.23±1.52,1.52±1.54,P<0.05);the ratio of CD8 in the model group was higher than that in the control group and experimental group(33.74%±2.46%vs 21.49%±2.07%,30.81%±2.27%,26.33%±2.05%,23.17%±2.15%,P<0.05).The contents of Scr,BUN and serum UA in the model group were higher than those in the control group and the experimental groups(Scr:68.62±5.74μmol/L vs 45.21±6.72μmol/L,62.17±7.23μmol/L,58.06±6.18μmol/L,52.45±6.85μmol/L,P<0.05;BUN:17.29±2.43mmol/L vs 7.26±2.01mmol/L,14.67±2.28m

关 键 词：大鼠 慢性肾小球肾炎 肾功能 炎症因子 免疫功能 黄葵胶囊 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]