翅果油亚微乳的制备及其抗疲劳活性研究  

Development of the Elaeagnus Mollis Oil Submicron-emulsion of Its Anti-fatigue Activity

作　　者：王晓敏[1] 白冰 李娟 张凯 谢茵[1] 荀波娜 毕小平[1] WANG Xiao-min;BAI Bing;LI Juan;ZHANG Kai;XIE Yin;XUN Bo-na;BI Xiao-pin(School of Pharmaceutical,Shanxi Medical University,Taiyuan 030001,Shanxi,China;Department of Neurology,First Hospital of Shanxi Medical University,Taiyuan 030001,Shanxi,China;Shanxi Qier Kang Samara Biological Products Co.,Ltd.,Taiyuan 030001,Shanxi,China;Shanxi DaYi Hospital,Taiyuan 030001,Shanxi,China)

机构地区：[1]山西医科大学药学院,山西太原030001 [2]山西医科大学第一医院神经内科,山西太原030001 [3]山西琪尔康翅果生物制品有限公司,山西太原030001 [4]山西大医院,山西太原030001

出　　处：《食品研究与开发》2018年第8期159-165,共7页Food Research and Development

基　　金：山西省服务产业创新学科群建设计划项目

摘　　要：制备一种安全有效,口感好的翅果油亚微乳,并探讨翅果油及其亚微乳抗疲劳活性及机理。以翅果油为原料,通过对乳化剂等组分进行处方筛选、采用正交设计进行工艺优化,制备翅果油亚微乳制剂,并对翅果油及其亚微乳抗疲劳活性进行研究。分别进行小鼠负重游泳实验,记录其力竭游泳时间,测定血乳酸(blood lactic acid,BLA)、血清尿素氮(serum urea nitrogen,BUN)和肝糖原(hepatic glycogen,LG)等指标含量,计算BLA、BUN的清除速率及LG的储备量。翅果油亚微乳的最优处方为:混合乳化剂(卵磷脂、聚甘油脂肪酸酯、单硬脂酸甘油酯)为4%、翅果油5%、甘油为4%,蒸馏水为76%,山梨酸钾为1%,蔗糖为10%。与阴性对照组比较,翅果油中剂量组负重游泳时间、BLA、BUN、LG等指标均具有统计学差异(P<0.05):高、低剂量组负重游泳时间及三项生化指标的均值略高于阴性对照组。亚微乳组各项指标均优于阴性组、翅果油低、高剂量组。成功研制出一种新型的,吸收好,生物利用度高的翅果油亚微乳。翅果油及其亚微乳具有一定的提高运动耐力,抑制运动后血乳酸和尿素氮积累、增加肝糖原储备的作用,从而达到缓解运动性体力疲劳的作用。The safe,effective and good tastes elaeagnus mollis oil submicron-emulsion was prepared.Furthermore,to study the anti-fatigue activity of elaeagnus mollis oil and its submicron-emulsion.The formulation of elaeagnus mollis oil submicron-emulsion was optimized by orthogonal-design,and the individual components include the emulsifier and the co-emulsifier for preparing are subjected to single factor screening.After treatment,the mice were subjected to weight-bearing swimming test.The blood lactic acid(BLA),serum urea nitrogen(BUN)and hepatic glycogen(LG)were measured immediately after swimming.The best formulation of the submirco-emulsion was as follows:mix emulsifier was 4%,oil was 5%,glycerol was 4%,distilled water 76%,potassium sorbate 1%and sucrose 10%.The loading swim time and the levels of BLA,BUN,LG in middle dose group were remarkably changed when compared with that of the control group(P<0.05).While there was no significant difference in the loading swim time and three biochemical indicators between the high/low-dose group and the control group.The clearance ratio of BLA and BUN in middle dose group were apparently higher than the control group.Conclusion:A new,safe,high bioavailability of elaeagnus mollis oil submicron-emulsion was developed successfully.Elaeagnus mollis oil and its submicron-emulsion has the effect of inhibiting the accumulation of BLA and BUN after exercise,increasing the content of LG,so as to alleviating the physical fatigue of mice.This work would provide a new perspective on developing of elaeagnus mollis oil.

关 键 词：翅果油 亚微乳 抗疲劳 小鼠 游泳 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]