补肾调经方和逍遥丸对超促排卵小鼠子宫内膜细胞Wnt通路的影响  

Effects of bushen tiaojing recipe and xiaoyao pill on Wnt pathway of endometrial cells in mice stimulated to ovulate

作　　者：杨丽芸 程长云 刘昱材 贾蕊 张瑞娟 杜惠兰 YANG Li-yun;CHENG Chang-yun;LIU Yu-cai;JIA Rui;ZHANG Rui-juan;DU Hui-lan(Acupuncture Massage School,Hebei University of Chinese Medicine,Shijiazhuang 050200,China;Chinese and Western Medicine School,Hebei University of Chinese Medicine,Shijiazhuang 050200,China;Pediatrics of Hebei Sanhe Hospital,Langfang 065200,China)

机构地区：[1]河北中医学院针灸推拿学院,石家庄050200 [2]三河市医院儿科,廊坊065200 [3]河北中医学院中西医结合学院,石家庄050200

出　　处：《中国医药生物技术》2018年第2期157-162,共6页Chinese Medicinal Biotechnology

基　　金：河北省中医药管理局项目(2016013);河北中医学院博士课题项目(7000120007)

摘　　要：目的观察补肾调经方和逍遥丸对超促排卵小鼠子宫内膜Wnt通路的影响。方法将90只雌性小鼠随机分为6组:补肾高、低剂量组,疏肝高、低剂量组,控制性超促排卵组(COH)和空白对照组,每组15只。各组每日腹腔注射生理盐水,连续8 d;第9天,除空白对照组腹腔注射生理盐水外,各组注射孕马血清,48 h后注射人绒毛膜促性腺激素。各组小鼠分别与雄鼠按1:1合笼,次晨观察妊娠情况后给药。补肾高、低剂量组分别给予补肾调经方口服液(5.4和2.7 g/ml),疏肝高、低剂量组分别给予逍遥丸混悬液(0.6和0.3 g/ml),COH组和空白对照组给予生理盐水,连续4 d。处死小鼠并取材,应用RT-PCR方法分别测定小鼠子宫Wnt7a和β-catenin的表达情况。结果与空白对照组比较,COH组Wnt7a和β-catenin表达降低;与COH组比较,补肾高剂量组和疏肝高剂量组Wnt7a和β-catenin表达增加(P<0.05);补肾高剂量组与疏肝高剂量组间比较无统计学意义(P>0.05)。结论补肾调经方、逍遥丸改善小鼠子宫内膜容受性,促进胚胎着床,其机制与上调Wnt7a和β-catenin表达水平,激活Wnt/β-catenin信号转导通路有关。Objective To investigate the influence of bushen treatment and shugan treatment on Wnt pathway of endometrial cells in mice stimulated to ovulate.Methods 90 mice were randomly divided into 6 groups:high and low dose of bushen group;high and low dose of shugan group;Super ovulation stimulated control group(COH)and blank control group,with each group having 15 mice.Mice in each group were given saline daily by intraperitoneal injection for 8 continuous days.On the 9th day,each group,except for the blank control group,was injected with PMSG,and 48 h later,the mice were given HCG.All the mice were mated with male mice according to 1:1 ratio,and were filled the stomach after examining female vaginal in the next morning.Bushen high and low dose group were given oral liquid filling kidney menstrual function party of 5.4 and 2.7 g/ml,respectively,while shugan high and low dose group were given xiaoyao pill for suspension liquid of 0.6 and 0.3 g/ml,respectively.COH group and the blank control group were given saline for 4 days.The expression of Wnt7a and beta-catenin mRNA in all the mice were measured by RT-PCR.Results Compared with the blank control group,the expression of Wnt7a andβ-catenin in the COH group were decreased(P<0.05).Compared with the COH group,the expression of Wnt7a andβ-catenin in the bushen high dose group and the shugan high dose group were increased(P<0.05).There was no statistically significant difference between these two high dose groups(P>0.05).Conclusion Bushen tiaojing recipe and xiaoyao pill can improve the endometrium tolerance of mice and promote embryo implantation.The mechanism is correlated to the increase in expression level of Wnt7a andβ-catenin and activation of Wnt/β-catenin signal transduction pathway.

关 键 词：补肾调经方 逍遥丸 Wnt7a β-catenin 

分 类 号：R285.5[医药卫生—中药学]