IL-32在不同分期哮喘患者血清中的表达比较  

A Comparative Study of IL-32 Concentration in Patients with Asthma of Various Phases

作　　者：王恋[1] 颜浩[1] 王玉珠[1] 何晨韵 吴驰 徐婷[1] WANG Lian;YAN Hao;WANG Yu-zhu;HE Chenyun;WU Chi;XU Ting(Department of Respiratory Diseases,the Second Hospital of Chengdu City,Chengdu,Sichuan 610017)

机构地区：[1]四川省成都市第二人民医院呼吸内科,610017

出　　处：《临床输血与检验》2018年第2期160-163,共4页Journal of Clinical Transfusion and Laboratory Medicine

基　　金：四川省卫生厅科研课题(No.120547)资助

摘　　要：目的探讨炎症细胞因子白细胞介素(IL)-32在哮喘患者血清中的表达及其意义。方法选择165例哮喘患者(包括急性发作期65例、慢性持续期42例及稳定期58例)作为研究对象,并以50例健康者为对照。采用酶联免疫吸附法检测血清IL-32。测定患者肺功能各指标、EOS数量、ECP水平和ACT评分,并分析IL-32与各指标的相关性。结果 IL-32(ng/L)在哮喘急性发作期组、慢性持续期组、稳定期组、健康对照组中的表达分别是53.87±20.07、43.48±7.17、29.55±6.26、13.68±4.16,差异有统计学意义(P<0.05)。FEV1、FEV1/FVC和PEF随病情的缓解而升高;EOS和ECP随病情的缓解而下降;ACT评分随病情的缓解越来越高。血清中IL-32的表达与FEV1、FEV1/FVC和ACT呈负相关,与EOS和ECP呈正相关,相关性具有统计学意义(P<0.05);血清中IL-32表达与PEF不具有相关性。结论 IL-32与哮喘的发生发展过程密切相关,动态监测患者血清中IL-32的表达可为患者的诊断、病情评估及治疗提供帮助。Objective To investigate the concentration and its significance of interleukin-32(IL-32)in sera of patients with asthma.Methods One hundred and sixty five cases of asthma were selected,including 65 cases of acute attack,42 cases of chronic asthma and 58 stable phases and 50 healthy controls.Enzyme linked immunosorbent assay was used to detect serum IL-32.The respiratory function,the number of EOS,ECP level,and ACT score were measured and the correlation between IL-32 and the serum indicators was analyzed.Results IL-32 concentrations were found to be 53.87+20.07 ng/L,43.48+7.17 ng/L,29.55+6.26 ng/L,and 13.68+4.16 ng/L in acute,chronic and stable asthma,and healthy control,respectively(P<0.05).FEV1,FEV1/FVC and PEF increased while EOS and ECP decreased with remission of the disease.ACT score became high with amelioration.The concentration of IL32 in sera was negatively correlated with FEV 1,FEV 1/FVC and ACT,but positively correlated with EOS and ECP(P<0.05).No correlation of IL32 level in the sera was noted with PEF.Conclusion IL-32 is closely related to the occurrence and advance of asthma.Monitoring concentration of IL-32 in the sera of patients may help for the diagnosis,treatment and prognosis of asthma.

关 键 词：白细胞介素-32 哮喘 预后 

分 类 号：R446.11[医药卫生—诊断学] R562.25[医药卫生—临床医学]