M蛋白在重氮法与钒酸氧化法检测胆红素中的影响初探  

To Learn the Influence of the Detemination of Bilirubin by Means of Diazo and Vanadate Oxidation in the Patient with Paraprotein

作　　者：徐云云 胡安群[1] XU Yun-yun;HU An-qun(Department of Clinical Laboratory,An qing City Affiliatted Hospital of Anhui Medical University 246003)

机构地区：[1]安徽医科大学附属安庆医院,246003

出　　处：《临床输血与检验》2018年第2期174-177,共4页Journal of Clinical Transfusion and Laboratory Medicine

摘　　要：目的通过对M蛋白阳性病例用重氮法、钒酸氧化法、干化学法测定胆红素浓度,探讨M蛋白在重氮法与钒酸氧化法胆红素测定中的影响。方法收集安庆市立医院2015年8月~2017年6月247例M蛋白阳性患者血清,分为多发性骨髓瘤(MM)组与非MM组,收集M蛋白阴性患者血清为对照组。三组均采用干化学法、重氮法、钒酸氧化法测定胆红素。以干化学检测结果为标准,对各组重氮法和钒酸氧化法胆红素的检测结果进行比较。结果 MM组和非MM组的组内重氮法和钒酸氧化法总胆红素检测结果的差异有统计学意义(P<0.05),而对照组的组内重氮法和钒酸氧化法总胆红素检测结果的差异无统计学意义(P>0.05)。MM组与对照组的重氮法与干化学法总胆红素检测结果的差值比较有统计学意义(P<0.05);非MM组与对照组重氮法与干化学法总胆红素检测结果的差值比较有统计学意义(P<0.05);MM组与非MM组重氮法与干化学法胆红素检测结果的差值比较有统计学意义(P<0.05)。MM组与对照组钒酸氧化法与干化学法总胆红素检测结果的差值比较有统计学意义(P<0.05);非MM组与对照组钒酸氧化法与干化学法总胆红素检测结果的差值比较有统计学意义(P<0.05);MM组与非MM组钒酸氧化法与干化学法总胆红素检测结果的差值比较无统计学意义(P>0.05)。结论 M蛋白影响重氮法和钒酸氧化法总胆红素测定,MM患者与非MM患者的M蛋白对重氮法胆红素测定的影响程度不同。Objective To learn the effect of paraprotein in bilirubin measured by diazo method,vanadate oxidation and dry chemistry through bilirubin concentration basing on in the patients with paraprotein.Methods The serum of 247 cases of patients with paraprotein positive from Anqing Municipal Hospital from August 2015 to June 2017 was collected and divided into treatment groups:multiple myeloma(MM)group and non MM group.The serum of 50 case of patients with negative paraprotein was collected as the control group.Each sample was analyzed by dry chemical method,diazonium method,and vanadate oxidation method..Analyse the test results of the determination of bilirubin in ways of diazo and vanadate compared with dry chemical.Results The difference of the bilirubin concentration by the means of diazonium and vanadate oxidation were statistically significant in the group of MM and non MM,while the difference were not statistically significant in the control group.The D-value of diazo method and dry chemistry were statistically significant in the MM group and control group,as in the groups of MM and non MM,meanwhile they were statistically significant in the non MM group and control group.The D-value of vanadate oxidation and dry chemistry were statistically significant in the MM group and control group,as non MM group and control group,while they were not statistically significant in the groups of MM and non MM.Conclusion The total bilirubin concentration was affected by diazotization and vanadate oxidation in patients with paraprotein,while paraprotein in patients with MM and non MM patients has different effects on the determination of bilirubin by diazo method.

关 键 词：M蛋白 胆红素 重氮法 钒酸氧化法 干化学法 影响 

分 类 号：R446.11[医药卫生—诊断学]