海洋真菌Westerdykella dispersa FS350次级代谢产物的分离纯化与鉴定  

Purification and identification of secondary metabolites from marine-derived fungus Westerdykella dispersa FS350

作　　者：许建林 刘洪新[2] 陈玉婵[2] 李赛妮 郭珩 李浩华[2] 章卫民[2] 高晓霞 XU Jianlin;LIU Hongxin;CHEN Yuchan;LI Saini;GUO Heng;LI Haohua;ZHANG Weimin;GAO Xiaoxia(School of Parmacy,Guangdong Pharmaceutical University,Guangzhou 510006,China;State Key Laboratory of Applied Microbiology Southern China,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,Guangdong Open Laboratory of Applied Microbiology,Guangdong Institute of Microbiology,Guangzhou 510070,China)

机构地区：[1]广东药科大学药学院,广东广州510006 [2]广东省微生物研究所/省部共建华南应用微生物国家重点实验室/广东省菌种保藏与应用重点实验室/广东省微生物应用新技术公共实验室,广东广州510070

出　　处：《广东药科大学学报》2018年第2期132-136,共5页Journal of Guangdong Pharmaceutical University

基　　金：广州市科技计划重点项目(21607020018);广东省自然科学基金研究团队项目(2016A030312014);国家自然科学基金项目(31272087);广东省海洋经济创新发展区域示范专项项目(GD2012-D01-002)

摘　　要：目的研究海洋真菌Westerdykella dispersa FS350的次级代谢产物。方法利用正相硅胶柱、反相硅胶柱、凝胶柱和高效液相色谱等多种色谱方法对菌株FS350的液体发酵产物进行分离纯化,采用NMR、MS等波谱方法鉴定化合物结构,采用SRB法和DPPH法测定化合物的生物活性。结果从发酵产物中分离得到了8个成分,分别鉴定为:2-(4-羟苯基)乙酸乙酯(1)、3-hydroxy-1-(2,6-dihydroxyphenyl)butan-1-one(2)、4-epiradicinol(3)、2-糠酸(4)、2-(4-羟苯基)-乙醇(5)、对羟基苯乙醇α-羟基丙酸酯(6)、(R)-甲羟戊酸内酯(7)和asterric acid(8)。结论化合物1~8均是首次从Westerdykella属真菌中分离得到,这些化合物均无明显的细胞毒和抗氧化活性。Objective To investigate the secondary metabolites from the marine fungus Westerdykella dispersa FS350.Methods The compounds were isolated from the fermentation broth of the marine fungus Westerdykella dispersa FS350 using various chromatographic methods including silica gel,reverse phase silica gel,Sephadex LH-20 and high performance liquid chromatography(HPLC),and their structures were determined by NMR and MS spectroscopic data analyses.The biological activities of the compounds were evaluated by SRB and DPPH methods.Results Eight constituents were isolated from the culture and identified as 2-(4-hydroxyphenyl)ethylacetate(1),3-hydroxy-1-(2,6-dihydroxyphenyl)butan-1-one(2),4-epiradicinol(3),2-furoic acid(4),2-(4-hydroxyphenyl)-ethanol(5),2-hydroxy-3-(4-hydroxyphenyl)propionic acid methyl ester(6),(R)-mevalonolactone(7)and asterric acid(8).Conclusion Eight compounds were isolated from the genus Westerdykella for the first time.No significant cytotoxic and antioxidant activities were detected for these compounds.

关 键 词：海洋真菌 Westerdykella dispersa 次级代谢产物 分离纯化 鉴定 

分 类 号：R282.2[医药卫生—中药学]