利用GBS-cpDNA揭示花生属花生区组内种间亲缘关系  

Reveal the Phylogenetic Relationship of Arachis Using GBS-cpDNA Data

作　　者：李春娟[1] 闫彩霞[1] 石程仁 赵小波[1] 王娟[1] 单世华[1] LI Chun-juan;YAN Cai-xia;SHI Cheng-ren;ZHAO Xiao-bo;WANG Juan;SHAN Shi-hua(Shandong Peanut Research Institute,Qingdao 266100,China)

机构地区：[1]山东省花生研究所,山东青岛266100

出　　处：《花生学报》2018年第1期38-42,共5页Journal of Peanut Science

基　　金：国家科技支撑计划项目(2014BAD11B04);山东省自然科学基金博士基金(ZR2017BC082);中央引导地方科技发展专项资金;花生抗逆;广适种质资源创新与利用(山东省农业良种工程);山东省现代农业产业技术体系花生遗传育种岗位(SDAIT-04-02)

摘　　要：揭示栽培种花生(Arachis hypogaea L.)与同属花生区组野生种之间的亲缘关系可为野生资源引入和遗传资源保存提供重要参考。本研究基于已公布的栽培种花生叶绿体全基因组序列,以11份花生区组的野生种资源和44份不同类型的栽培种花生为研究材料,利用GBS(Genotyping-by Sequencing)测序结果中能够比对到叶绿体全基因组的序列信息,获得了叶绿体基因组上111个SNP位点和10个InDel位点。根据系统演化树以及主成分判别分析结果显示:与栽培种花生关系最近的野生种是A.monticola,较近的是A.duranensis、A.batizocoi、A.paraguariensist和A.hoehnei,较远的野生种包括A.helodes、A.cardenasii、A.villosa、A.stenosperma。花生区组种间亲缘关系的阐明,对花生远缘杂交育种具有重要意义。To reveal the phylogenetic relationship among the cultivated peanut and congeneric species is the foundation for the introduction and the preservation of genetic resources.The first chloroplast genome sequence of cultivated peanut has been published since 2016 and therefore provided important reference for this study.The GBS-cpDNA data was extracted to detect the genetic varieties.A total of 111 SNPs and 10 InDels was used to build phylogenetic tree and PCA analysis.The results indicated that the closely related wild species is A.monticola.The furthest relationship included A.helodes,A.cardenasii,A.villosa and A.stenosperma,which provided us a better understanding of the phylogenetic relationships among species of sec.Arachis and a prerequisite for the effective use of these gene pools.

关 键 词：GBS-cpDNA 花生 亲缘关系 系统树 

分 类 号：S565.2[农业科学—作物学] S324