宁夏主要水稻品种(系)Rf-1基因位点多态性分析  

Polymorphism Analysis of Rf-1 Gene Locus of Main Rice Varieties(Lines) in Ningxia

作　　者：王坚[1] 刘炜[1] WANG Jian;LIU Wei(The Crop Research Institute of Ningxia Academy of Agricultural and Forestry Sciences,Yongning,Ningxia 750105,China)

机构地区：[1]宁夏农林科学院农作物研究所,宁夏永宁750105

出　　处：《西北植物学报》2018年第3期456-464,共9页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(31160273);宁夏自然科学基金(NZ14185);自治区农业育种专项水稻新品种选育项目(2013NYY20305)

摘　　要：以宁夏49份主要水稻品种(系)为实验材料,对各材料在Rf-1基因位点的功能标记进行分析和基因序列对比,并对各材料在不同位点的差异进行聚类分析,以明确Rf-1位点的规律和特证,为不育系或恢复系的转育及其分子标记选用、杂交组合配置和杂种优势的利用提供理论参考。结果表明:(1)有11个品种(系)为Rf1a/Rf1a基因型,占宁夏主要水稻品种(系)的22%。(2)Rf1b基因位点检测结果有17个点的碱基发生突变,其中2459、2283、2059、1694、2205、1784、2315位点在各品种(系)中突变的频率较高,1576、1881、1970(1985)位点突变的频率低。(3)突变主要是碱基的替换,其位点多,品种(系)间变化大;插入突变位点相对少,只有极少数品种(系)发生插入突变;各品种(系)平均有8个突变位点。(4)聚类分析发现,宁夏主要水稻品种(系)可以划分为三类,每类对应了不同时期的主要品种(系),从第一类群体到第三类群体品种(系)间的差异增加,多样性更丰富,但平均每个品种突变点由9个下降到7个。49 samples selected from main varieties(line)of rice from Ningxia are taken as experimental material,the functional marker of which are subject to analysis and gene sequence comparison at Rf-1 gene locus and discrepancies in all materials at various loci are subject to cluster analysis to determine pattern and characteristic of Rf-1 locus and offer theoretical reference for trans-breeding,molecular marker selection,configuration of hybrid combination and utilization of hybrid vigor of sterile line and restore line.The results show that:(1)11 varieties(lines)are(Rf1a/Rf1a)genetype,accounting for 22%of main rice variety(line)in Ningxia;(2)Rf1b gene locus detection found mutation at basic group of 17 points,among which loci 2459,2283,2059,1694,2205,1784 and 2315 have the highest frequency of mutation among each variety(line),while loci 1576,1881,1970(1985)have the lowest frequency of mutation;(3)The mutations are mainly replacement of nucleic acid characterized by numerous loci,including large variation among varieties(lines)and relevant less insertion mutation locus,only a few varieties(lines).There are about 8 mutation loci in each variety(Line)in average;(4)It is observed from cluster analysis that main rice varieties(lines)in Ningxia could be divided into 3 types,with each type corresponding to main varieties(lines)at different periods,difference of varieties(lines)from the first cluster to the third cluster grows and becomes more diverse,but mutation of each variety decreased from 9 to 7 in average.

关 键 词：宁夏水稻 雄性不育恢复基因 功能标记 基因序列 

分 类 号：Q789[生物学—分子生物学]