植物血凝素刺激外周血单个核细胞Th1/Th2细胞因子分泌对阿尔茨海默病的诊断价值  

Th1/Th2 cytokines secreted by phytohaemagglutinin-stimulated peripheral blood mononuclear cells for the diagnosis of Alzheimer's disease

作　　者：丁冰玉[1] 林萍[2] 李擎天[1] DING Bingyu;LIN Ping;LI Qingtian(Faculty of Laboratory Medicine,Ruijin Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 200025,China;Shanghai Mental Health Center,Shanghai Jiaotong University School of Medicine,Shanghai 200030,China)

机构地区：[1]上海交通大学医学院附属瑞金医院检验系,上海200025 [2]上海交通大学医学院附属精神卫生中心,上海200030

出　　处：《检验医学》2018年第4期290-294,共5页Laboratory Medicine

摘　　要：目的探讨阿尔茨海默病(AD)患者外周血单个核细胞(PBMC)在植物血凝素(PHA)刺激下分泌的细胞因子、血清细胞因子以及血脂水平对疾病的诊断价值。方法收集AD患者血液样本100例以及健康对照者血液样本100例,抽提PBMC,在PHA刺激下培养48 h,采用双抗体夹心酶联免疫吸附试验(ELISA)测定培养液上清中细胞因子白细胞介素(IL)-1α、IL-6、IL-10、肿瘤坏死因子α(TNF-α)以及γ干扰素(IFN-γ)水平;同时检测血清IL-1α(sIL-1α)、血清IL-6(sIL-6)、血清IL-10(sIL-10)、血清TNF-α(sTNF-α)及血清IFN-γ(s IFN-γ)水平和血脂[总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)及低密度脂蛋白胆固醇(LDL-C)]水平。6周后AD患者重新采样检测。结果 AD组PBMC分泌的IL-1α、IFN-γ水平明显高于对照组(P<0.05),而IL-10水平明显低于对照组(P<0.05);AD组sIL-6及sTNF-α水平明显高于对照组(P<0.05);AD组血清TC、TG及LDL-C水平明显高于对照组(P<0.05)。AD组6周后重新采样的检测结果与第1次一致。结论在PHA刺激下PBMC的IFN-γ等细胞因子分泌水平、血清细胞因子水平及血脂水平异常可能与AD发病相关,其中PHA刺激的IFN-γ及sIL-6、sTNF-α、TC、TG、LDL-C水平检测对AD有一定的诊断价值。Objective To investigate the roles of cytokines,serum cytokines and blood lipid secreted by phytohaemagglutinin(PHA)-stimulated peripheral blood mononuclear cells(PBMC)for the diagnosis of Alzheimer's disease(AD).Methods A total of 100 AD patients and 100 healthy subjects were enrolled,and their blood samples were collected.PBMC was extracted.The levels of culture solution cytokine interleukin(IL)-1 alpha,IL-6,IL-10,tumor necrosis factor alpha(TNF-α)and interferon gamma(IFN-γ),serum cytokines(sIL-1α),sIL-6,sIL-10,sTNF-αand sIFN-γand blood lipid[total cholesterol(TC),triglyceride(TG),high-density lipoprotein cholesterol(HDL-C)and low-density lipoprotein cholesterol(LDL-C)]levels were determined by enzyme-linked immunosorbent assay(ELISA).Samples were collected and determined again after 6 weeks.Results The levels of IL-1αand IFN-γin AD group were higher than those in control group(P<0.05),while the level of IL-10 in AD group was lower than that in control group(P<0.05).The levels of sIL-6 and sTNF-αwere higher in AD group than those in control group(P<0.05).The levels of TC,TG and LDL-C in AD group were higher than those in control group(P<0.05).After 6 weeks,the results of re-sampling were similar those of the first determination.Conclusions Cytokines,serum cytokines and blood lipid secreted by PHA-stimulated PBMC,such as IFN-γ,may be associated with AD.Among them,IFN-γ,sIL-6,sTNF-α,TC,TG and LDL-C may be potential for the diagnosis of AD.

关 键 词：细胞因子 植物血凝素 外周血单个核细胞 阿尔茨海默症 

分 类 号：R446.62[医药卫生—诊断学]