CFW荧光染色法检测疑似真菌感染的临床应用评价  

CFW fluorescent staining in detecting suspected fungal infection

作　　者：何文婧 高志琴 陈健 任弘谨 杨虹 蔡晴 杨连娟 HE Wenjing;GAO Zhiqin;CHEN Jian;REN Hongjin;YANG Hong;CAI Qing;YANG Lianjuan(Shanghai Dermatology Hospital,Shanghai 200050,China)

机构地区：[1]上海市皮肤病医院,上海200050

出　　处：《检验医学》2018年第4期331-334,共4页Laboratory Medicine

基　　金：上海市卫生和计划生育委员会面上项目(20140388)

摘　　要：目的探讨荧光染色剂Calcofluor White(CFW)在真菌感染诊断中的临床应用价值,并与传统KOH湿片镜检法及真菌培养法进行比较,寻找更有效的真菌检测方法。方法选取临床155例疑似真菌感染的患者,采用KOH湿片镜检法、CFW荧光染色法和真菌培养法进行检测。结果 CFW荧光染色法阳性率(85.8%)最高,KOH湿片镜检法阳性率(76.1%)次之,二者差异有统计学意义(χ2=14.45,P<0.05),真菌培养法阳性率(71.0%)最低。结论 CFW荧光染色法可显著提高真菌检测的阳性率,是一种有效的真菌检测方法。Objective To investigate the role of fluorescent dye Calcofluor White(CFW)in the diagnosis of fungal infection,and to compare with KOH microscopic examination and fungal culturing,so as to find an effective method for detecting fungi.Methods A total of 150 clinically suspected patients with fungal infection were enrolled,and the samples were detected by CFW,KOH microscopic examination and fungal culturing.Results The positive rate of CFW was 85.8%,the positive rate of KOH microscopic examination and fungal culturing were 76.1%and 71.0%,respectively.There was statistical significance between CFW and KOH microscopic examination(χ2=14.45,P<0.05).Conclusions CFW could improve positive rate,and it is an effective method for detecting fungi.

关 键 词：荧光染色剂Calcofluor WHITE KOH 真菌培养 

分 类 号：R446.1[医药卫生—诊断学]