RhoA/ROCK信号通路在先天性巨结肠病变肠段的变化研究  

Variation of Rho A/ROCK signaling pathway in colon of Hirschsprung's disease

作　　者：童玲 臧婧羽 袁小建 洪志华[3] 邬文杰[2,3] 陈杰 许文燮[1,2] Tong Ling;Zang Jingyu;Yuan Xiaojian;Hong Zhihua;Wu Wenjie;Chen Jie;Xu Wenxie(Department of Anatomy&Physiology,Shanghai Jiaotong University School of Medicine,Shanghai 200240,China;Department of Pediatric Surgery,Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine,Shanghai 200092,China;Department of Pediatric Surgery,Jiaxing Maternity and Child Health Care Hospital(Department of Pediatric Surgery,Xinhua Hospital,Jiaxing Branch),Jiaxing 314000,China)

机构地区：[1]上海交通大学医学院基础医学院解剖与生理学系,上海市200240 [2]上海交通大学医学院附属新华医院小儿外科,上海市200092 [3]嘉兴市妇幼保健院小儿外科(上海新华医院小儿外科嘉兴分部),浙江省嘉兴市314000

出　　处：《临床小儿外科杂志》2018年第3期226-230,共5页Journal of Clinical Pediatric Surgery

基　　金：国家自然科学基金(31671192);新华医院院级科研课题项目(JZPI201708)

摘　　要：目的观察在先天性巨结肠(Hirschsprung's disease,HD)病变肠段中,与Rho A/ROCK信号通路相关的蛋白的表达情况,为HD患者结肠动力障碍提供新的研究角度。方法采用Western blotting的方法,分别对9例HD患儿手术切除的病变段和扩张段肠壁中与Rho A/Rho激酶(Rho K,ROCK)信号通路相关蛋白的表达进行检测。结果 9例患者狭窄段P(Ser19)-MLC20的表达水平与扩张段相比明显减少,差异有统计学意义(t=2.527,P=0.035);在与Rho A/ROCK信号通路相关的蛋白表达中,狭窄段ROCK1蛋白表达与扩张段相比明显减少(t=3.104,P=0.015),狭窄段ROCK2蛋白表达与扩张段相比减少(t=2.357,P=0.046),狭窄段P(Thr853)-MYPT1蛋白表达与扩张段相比也减少(t=2.857,P=0.028),差异均有统计学意义。结论 HD患者病变部位平滑肌细胞的Rho A/ROCK信号通路明显减弱,说明该途径可能与HD的发病过程相关。Objective To explore the protein expression of RhoA/ROCK signaling pathway in Hirschsprung s disease(HD)and provide new perspectives for studying colonic dysfunction in HD patients.Methods The protein expression involved in RhoA/ROCK signaling pathway was examined by Western blot in 9 HD cases aged from 6 months to 2 years.And statistical software was used for analyzing the results.Results The expression of P(Ser19)-MLC 20 obviously decreased in narrow segment as compared with dilated counterpart(t=2.527,P=0.035);and regarding the protein expressions involved in RhoA/ROCK signaling pathway,the expression of ROCK1 obviously decreased in narrow segment as compared with dilated counterpart(t=3.104,P=0.015).The expression of ROCK2 decreased in narrow segment compared with dilated counterpart(t=2.357,P=0.046).The expression of P(Thr853)-MYPT1 also decreased in narrow segment as compared with dilated counterpart(t=2.857,P=0.028).And both had statistical significance.Conclusion RhoA/ROCK signaling pathway is down-regulated in HD.And these results may be related to colonic dysfunction of HD patients.

关 键 词：HIRSCHSPRUNG病 RHO激酶 肌细胞 平滑肌 收缩 结肠 

分 类 号：R726.5[医药卫生—儿科]