皖南蝮蛇抑瘤组分Ⅰ对人胃癌SGC-7901细胞作用机制的初步探讨  

Preliminary study on the mechanism of Agkistrodon halys venom antitumor component-Ⅰ in human gastric cancer SGC-7901 cells

作　　者：赵容[1] 卢林明[2] 张根葆[1] 柴琳 周珏[2] ZHAO Rong;LU Linming;ZHANG Genbao;CHAI Lin;ZHOU Jue(Department of Pathology,Wannan Medical College,Wuhu 241002,China)

机构地区：[1]皖南医学院病理生理学教研室,安徽芜湖241002 [2]皖南医学院病理学教研室,安徽芜湖241002 [3]皖南医学院口腔医学院,安徽芜湖241002

出　　处：《皖南医学院学报》2018年第2期113-115,共3页Journal of Wannan Medical College

基　　金：安徽省高校省级自然科学重点项目(KJ2014A276)

摘　　要：目的:探讨皖南蝮蛇抑瘤组分Ⅰ(AHVAC-Ⅰ)对人胃癌SGC-7901细胞作用的基本机制。方法:本实验选择SGC-7901细胞作为研究对象,体外条件下传代培养。按实验目的分为正常对照组和AHVAC-Ⅰ浓度组。MTT法检测不同浓度的AHVAC-Ⅰ对SGC-7901细胞的增殖抑制作用,通过化学荧光法对细胞内线粒体膜电位(△Ψm)分析。结果:AHVAC-Ⅰ能抑制SGC-7901细胞增殖,在一定范围内(3~30 mg/L)呈剂量依赖性,引起SGC-7901细胞出现死亡并伴随有染色质聚集、核破碎、凋亡小体等典型的凋亡形态学特征;此外还检测到有△Ψm降低现象。结论:AHVAC-Ⅰ对SGC-7901细胞的作用与线粒体凋亡途径有关,作用机制之一是通过降低△Ψm诱导人胃癌SGC-7901细胞凋亡。Objective:To investigate the primary mechanisms of Agkistrodon halys venom antitumor component-Ⅰ(AHVAC-Ⅰ)in human gastric cancer cell line SGC-7901.Methods:SGC-7901 cells were used,and sub-cultured with in vitro technique.The experiment was performed as normal control group and different AHVAC-Ⅰconcentration treatment group.MTT method was used to examine the inhibitory effects of different concentrations of AHVAC-Ⅰon SGC-7901 cell proliferation,and the changes of mitochondrial membrane potential(△Ψm)were detected by chemiluminescence method.Results:MTT determination showed that the inhibition rate of cell proliferation was increased with added AHVAC-Ⅰconcentration(3-30 mg/L),which resulted in SGC-7901 cell death with chromatin aggregation,nuclear fragmentation,apoptotic bodies and other typical apoptotic morphological features.Additionally,decreased mitochondrial membrane potential was observed.Conclusion:AHVAC-Ⅰaffecting SGC-7901 cells is associated with mitochondrial apoptosis pathway.One of the potential mechanisms is to induce apoptosis of human gastric cancer SGC-7901 cells via reducing the mitochondrial membrane potential.

关 键 词：胃癌 蛇毒 凋亡 线粒体途径 

分 类 号：R329.25[医药卫生—人体解剖和组织胚胎学] R735.2[医药卫生—基础医学]