绿竹侧枝扦插生根机理研究  

Experiment on Rooting of Dendrocalamopsis oldhami Cuttings

作　　者：凡莉莉 薛磊 荣俊冬[1] 姚旺[1] 郑郁善[1] 苏小青[1] FAN Li-li;XUE Lei;RONG Jun-dong;YAO Wang;ZHENG Yu-shan;SU Xiao-qing(College of Forestry,Fujian Agriculture and Forestry University,Fuzhou 350002,China;College of Landscape,Fujian Agriculture and Forestry University,Fuzhou 350002,China)

机构地区：[1]福建农林大学林学院,福建福州350002 [2]福建农林大学园林学院,福建福州350002

出　　处：《浙江林业科技》2018年第1期20-26,共7页Journal of Zhejiang Forestry Science and Technology

基　　金：福建省区域发展项目(2015N3015);福建省科技重大专项(2013NZ0001)资助

摘　　要：为了探明生长素处理对绿竹Dendrocalamopsis oldhami扦插苗生根机理的影响,为绿竹扦插育苗提供理论依据,以2年生绿竹粗壮侧枝为实验材料,采用600 mg·L^(-1)的ABT1号先处理5 min,以黄泥土:泥炭土(1:1)为扦插基质,进行扦插试验,研究绿竹插条生根特性和生根过程中可溶性糖、可溶性蛋白含量和相关抗氧化酶活性变化。结果表明:600 mg·L^(-1)的ABT1号处理对扦插生根具有影响,处理后的插条生根率可达57.25%;绿竹扦插生根属于潜伏不定根原基生根型,具体过程分为根的诱导时期(0~14 d)、表达时期(14~21 d)和伸长时期(21~35 d)3个阶段;在扦插生根过程中,可溶性糖含量变化呈下降-上升-下降趋势,可溶性蛋白含量变化呈上升-下降-上升趋势;在不定根诱导期(0~14 d),过氧化物酶(POD),吲哚乙酸氧化酶(IAAO),多酚氧化酶(PPO)和超氧化物歧化酶(SOD)活性呈上升趋势;在表达期(14~21 d),POD和PPO活性下降,IAAO活性上升,SOD活性保持稳定;在伸长期(21~35 d),IAAO和PPO活性下降,POD活性先上升后下降,SOD活性略上升。In November 2015,collections of lateral branches of Dendrocalamopsis oldhami were carried out in Dongshan county,Zhangzhou of Fujian province for experiment of rooting of cuttings.Cuttings were treated with 600 mg/L of ABT1 for 5 min,and planted in medium of yellow soil:peat soil=1:1.Determinations and observation were implemented on rooting properties and changes of soluble sugar content,soluble protein content,and some antioxidant enzymes activity during rooting time.The results showed that treated cuttings had rooting rate of 57.25%.During the process of rooting,the content of soluble sugar decreased first,then increased,and decreased at last.The changes of soluble protein content showed the opposite trend.The activity of peroxidase(POD),indole acetic acid oxidase(IAAO),polyphenol oxidase(PPO)and superoxide dismutase(SOD)increased during 0-14th day after planting,and the activity of POD and PPO decreased,that of IAAO increased while that of SOD stable during the 14th-21st day.Activities of IAAO and PPO declined,of POD increased first and then decreased and of SOD slightly increased during the 21st-35th day.

关 键 词：绿竹 扦插生根 生根机理 营养物质 抗氧化酶 

分 类 号：S795.5[农业科学—林木遗传育种]