功能化纳米金目视检测铅  

The Visual Assay for Lead Based on the Multi-functionalized Gold Nanoparticles

作　　者：刘欢 白拖 曹婉婷 徐梦妮 黄婷 LIU Huan;BAI Tuo;CAO Wanting;XU Mengni;HUANG Ting(School of Chemistry&Chemical Engineering,Xi’an Shiyou University,Xi’an 710065,Shaanxi,China;School of Chemistry&Chemical Engineering,Ankang University,Ankang 725000,Shaanxi,China)

机构地区：[1]西安石油大学化学化工学院,陕西西安710065 [2]安康学院化学化工学院,陕西安康725000

出　　处：《安康学院学报》2018年第2期95-98,共4页Journal of Ankang University

基　　金：陕西省教育厅大学生创新项目(2015sxjy023;2016sxjy001);陕西省教育厅科研计划项目(15JK1004);陕西省科技厅协同创新计划项目(2015XT-63)

摘　　要：本文使用一种简单、快速且具有选择性的测试方法,检测水样中的Pb^(2+)。该方法基于"17DS-17E"脱氧核酶、条形码DNA的纳米金结合物和捕获DNA-BSA轭合物,将其分别涂在控制区和测试区显色并进行定性分析和检测。结果表明,由于17E脱氧核酶对Pb^(2+)具有高效的特异性,Pb^(2+)的存在促使17E酶链分裂底链17DS,Pb^(2+)的测试结果可以在10 min内获得,检出限为10 nmol/L。该传感系统具有良好的选择性,其他二价金属离子对传感没有明显的干扰,且试纸条能长期使用,该测试条是一种潜在的现场快速检测Pb^(2+)的筛查工具。This study uses a simple,rapid and selective test method,detection of lead(II)in samples.The method is based on the“17DS-17E”ribozyme,the gold nanoparticle conjugates of barcode DNA and the capture of DNA-BSA conjugates,which are respectively coated in the control area and the color of the test area for qualitative analysis and detection.The results show that the 17E deoxyribozyme has high specificity for lead(II)which has prompted 17E enzyme chain split bottom chain 17DS,test results can be obtained within 10 min,the detection limit was 10 nmol/L.The sensor system has good selectivity,other two valence metal ions have no obvious interference to the sensor and the test strip can be used for a long time.This test strip is a potential screening tool for rapid detection of lead(II)in the field.

关 键 词：铅离子 纳米金结合物 捕获DNA-BSA轭合物 测试条 

分 类 号：O61[理学—无机化学]