小檗碱对糖尿病大鼠心血管的保护作用  

Protective Effects of Berberine on the Cardiovascular in Diabetic Rats

作　　者：芦琨[1] 赫金凤 常景芝[1] 胡灵卫[1] 田华[1] LU Kun;HE Jinfeng;CHANG Jingzhi;HU Lingwei;TIAN Hua(Department of Biochemistry,Shangqiu Medical College,Shangqiu 476100,China;Department of Clinical Medicine,Shangqiu Medical College,Shangqiu 476100,China)

机构地区：[1]商丘医学高等专科学校生物化学教研室,商丘476100 [2]商丘医学高等专科学校临床医学系,商丘476100

出　　处：《医药导报》2018年第5期518-522,共5页Herald of Medicine

基　　金：河南省商丘市科技发展计划项目(153062)

摘　　要：目的观察小檗碱对糖尿病大鼠血糖、血脂以及心血管病变相关因子的影响,探讨其可能的作用机制。方法采用腹腔注射链脲佐菌素(STZ)复制糖尿病大鼠模型,将造模成功的43只大鼠随机分为模型对照组(n=9)、二甲双胍组(140 mg·kg^(-1)·d^(-1),n=8)和小檗碱小、中、大剂量组(50,100,150 mg·kg^(-1)·d^(-1),n=9,9,8)。另取正常大鼠8只,作为正常对照组。灌胃给药12周后,取大鼠心脏,计算心脏质量指数;试剂盒测定血清中三酰甘油(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)和一氧化氮(NO)含量;放射分析法测定大鼠血清中血栓素B_2(TXB_2)、6-酮-前列腺素Fα(6-keto-PGFα)和内皮素(ET)的含量;实时荧光定量-聚合酶链反应(RT-PCR)检测大鼠胸主动脉组织细胞间黏附分子-1(ICAM-1)和血管内皮细胞黏附分子-1(VCAM-1)的表达。结果与模型对照组比较,小檗碱中、大剂量组和二甲双胍组血糖水平、心脏质量指数、TG、TC、LDL-C的含量明显降低(P<0.01);血清中NO、6-keto-PGFα含量明显升高(P<0.01),ET和TXB_2含量明显下降(P<0.01);胸主动脉组织ICAM-1和VCAM-1 mRNA的表达均明显降低(P<0.01)。结论小檗碱可通过调节血脂修复糖尿病大鼠血管收缩与舒张的动态平衡,拮抗动脉粥样硬化,从而对心血管起到一定程度的保护作用。Objective To investigate the possible mechanism of berberine on the cardiovascular in diabetic rats by observing the effect of berberine on the blood glucose,blood lipid and factors involved in cardiovascular injury.Methods The diabetic model was induced by intraperitoneal injection of streptozotocin(STZ).The 43 rats of the model were randomly divided into model control group(n=9),metformin group(n=8)and berberine low,medium,high dose groups(50,100,150 mg·kg-1·d-1,n=9,9,8).Normal rats(n=8)were set as normal control group.After 12 weeks of gavage administration,rats’heart was taken and heart mass index(HMI)was calculated;concentration of TG,TC,HDL-C,LDL-C,NO was examined by kit;rats’serum concentration of blood TXB 2,6-keto-PGFαand ET was determined by radioimmunoassay;the expression of ICAM-1and VCAM-1 in thoracic aorta tissue was tested by real-time quantitative PCR(RT-PCR).Results Compared with the model control group,levels of BG,HMI,TG,TC,LDL-C were significantly decreased(P<0.01)in berberine medium,high dose groups and metformin group;the serum concentration of NO and 6-keto-PGFαwas increased(P<0.01)while ET and TXB 2 was decreased(P<0.01);the expression of ICAM-1and VCAM-1 in thoracic aorta tissue was decreased obviously(P<0.01).Conclusion Berberine has protective effects on the cardiovascular in diabetic rats by means of regulating blood lipid,recovering blood vessel’s dynamic balance of systolic and diastolic,playing a role in anti-atherosclerosis.

关 键 词：小檗碱 糖尿病 心血管疾病 血管内皮 

分 类 号：R282.71[医药卫生—中药学] R285.5[医药卫生—中医学]