多管藻中R-藻蓝蛋白与别藻蓝蛋白的分离纯化  

Isolation and Purification of R-phycocyanin and Allophycocyanin from Marine Red Alga Polysiphonia urceolata

作　　者：王胜楠 刁海平 赵明日[1] 孙力[1] WANG Sheng-nan;DIAO Hai-ping;ZHAO Ming-ri;SUN Li(School of Life Sciences,Yantai University,Yantai 264000,China)

机构地区：[1]烟台大学生命科学学院,山东烟台264000

出　　处：《烟台大学学报（自然科学与工程版）》2018年第2期105-113,共9页Journal of Yantai University(Natural Science and Engineering Edition)

基　　金：国家自然科学基金资助项目(40976083)

摘　　要：以海生大型红藻多管藻(Polysiphonia urceolata)为材料,建立了从多管藻藻胆蛋白提取液中有效分离纯化藻蓝蛋白(R-PC)和别藻蓝蛋白(AP)的层析技术方法,即先使用Sephacryl S-300(S-300)和Sephacryl S-200(S-200)2种凝胶过滤层析从藻胆蛋白提取液中获得R-PC/AP组分,再依次经过Q Sepharose Fast Flow离子交换层析和Phenyl Sepharose 6 Fast Flow疏水层析对凝胶过滤获得的R-PC/AP组分进一步纯化.An optimized technical procedure for simultaneous preparation of the R-phycocyanin(R-PC)and allophycocyanin(AP)from the extracted phycobiliprotein solution of marine red alga Polysiphonia urceolata is established based on the present experiments.R-PC and AP are isolated from the extracted phycobiliprotein solution of Polysiphonia urceolate using gel filtration through Sephacryl S-300(S-300)and S-200(S-200).The obtained fractions of R-PC and AP are further purther purified using ion exchange chromatography through Q Sepharose Fast Flow,then by using hydrophobic interaction chromatography through Phenyl Sepharose 6 Fast Flow.

关 键 词：凝胶过滤 离子交换 疏水层析 多管藻 藻蓝蛋白 别藻蓝蛋白 

分 类 号：Q503[生物学—生物化学]