当归芍药散含药血清抑制内皮素-1介导的肝星状细胞收缩的机制研究  

Mechanism of Action of Serum Containing Danggui Shaoyao San in Inhibiting Hepatic Stellate Cell Contraction Mediated by Endothelin-1

作　　者：刘浩坤[1] 潘永福[1] 周敏[1] 王运来[1] 尹丹丹 许钒[1] LIU Hao-kun;PAN Yong-fu;ZHOU Min;WANG Yun-lai;YIN Dan-dan;XU Fan(Anhui University of Chinese Medicine,Anhui Hefei 230012,China)

机构地区：[1]安徽中医药大学,安徽合肥230012

出　　处：《安徽中医药大学学报》2018年第2期76-80,共5页Journal of Anhui University of Chinese Medicine

基　　金：国家自然科学基金项目(81173368;81573720)

摘　　要：目的探讨当归芍药散(Danggui Shaoyao San,DSS)含药血清对内皮素-1(endothelin,ET-1)介导的肝星状细胞内皮型一氧化氮合酶(endothelial nitric oxide synthase,eNOS)和RhoA/ROCKⅡ蛋白表达的影响。方法将实验动物分为空白组和给药组,分别提取血清,-20℃冻存备用。细胞实验分为6组:空白组,模型组,DSS含药血清高、中、低剂量组,Y-27632抑制剂组。肝星状细胞培养24h后,采用Western blot法检测细胞内RhoA、ROCKⅡ、eNOS蛋白的表达水平。结果与空白组比较,模型组RhoA、ROCKⅡ蛋白表达水平均显著升高(P<0.05),细胞内eNOS蛋白表达水平明显下降(P<0.05);与模型组比较,DSS含药血清各剂量组RhoA、ROCKⅡ蛋白表达水平减少(P<0.05),DSS含药血清高、中剂量组细胞内eNOS蛋白表达水平增多(P<0.05)。结论 DSS可通过抑制肝星状细胞RhoA、ROCK蛋白的表达,上调eNOS蛋白的表达,抑制ET-1诱导的肝星状细胞收缩。Objective To investigate effect of serum containing Danggui Shaoyao San(DSS)on the protein expression of endothelial nitric oxide synthase(eNOS),ras homolog gene family,member A(RhoA),and Rho-associated kinaseⅡ(ROCKⅡ)in hepatic stellate cells(HSCs)in response to endothelin-1(ET-1).Methods Experimental rats were divided into normal group and serum containing DSS group,and serum was collected and stored at a temperature of-20℃for future use.There were six groups in the cell experiment,i.e.,blank group,model group,low-,medium-,and high-dose serum containing DSS groups,and Y-27632 inhibitor group.After HSCs were cultured for 24 hours,Western blot was used to measure the protein expression of RhoA,ROCKⅡ,and eNOS in HSCs.Results Compared with the blank group,the model group had significant increases in the protein expression of RhoA and ROCKⅡ(P<0.05)and a significant reduction in the protein expression of eNOS(P<0.05).Compared with the model group,the low-,medium-,and high-dose serum containing DSS groups had significant reductions in the protein expression of RhoA and ROCKⅡ(P<0.05),and the medium-and high-dose groups had a significant increase in the protein expression of eNOS(P<0.05).Conclusion DSS can suppress the protein expression of RhoA and ROCK and upregulate the protein expression of eNOS,thus inhibiting HSC contraction mediated by ET-1.

关 键 词：当归芍药散 肝星状细胞 RHOA ROCKⅡ 内皮型一氧化氮合酶 

分 类 号：R285.5[医药卫生—中药学]