不同产地知母须根中3个酮类成分含量测定  

Content Determination of Three Xanthones in the Fibrous Root of Anemarrhena asphodeloides from Different Producing Areas

作　　者：贾鹏晖 黄琪 许凤清 金传山 王培 马凯 穆凤扬 吴德玲 JIA Peng-hui;HUANG Qi;XU Feng-qing;JIN Chuan-shan;WANG Pei;MA Kai;MU Feng-yang;WU De-ling(School of Pharmacy,Anhui University of Chinese Medicine,Anhui Hefei 230012,China;Anhui Key Laboratory for Modern Chinese Materia Medica,Anhui Hefei 230012,China;Bozhou Yong Gang Decoction Factory Co.,Ltd.,Anhui Bozhou 236800,China)

机构地区：[1]安徽中医药大学药学院,安徽合肥230012 [2]安徽省现代中药重点实验室,安徽合肥230012 [3]亳州市永刚饮片厂有限公司,安徽亳州236800

出　　处：《安徽中医药大学学报》2018年第2期90-92,共3页Journal of Anhui University of Chinese Medicine

基　　金：中医药行业科研专项项目(201507002-18);安徽省高校科研创新平台团队(中药饮片产地加工与炮制一体化关键技术研究创新团队)建设项目(2015TS035);安徽省科技攻关项目(150104117)

摘　　要：目的测定不同产地知母须根中新芒果苷、芒果苷和异芒果苷的含量。方法采用高效液相二极管陈列检测法,Shim-pack色谱柱(4.6mm×250mm,5μm);乙腈-0.4%冰醋酸水溶液梯度洗脱,流速1.0mL/min;柱温25℃;检测波长为258nm。结果所收集的知母须根样品中新芒果苷的含量为1.2%~1.9%,芒果苷的含量为1.0%~1.6%,异芒果苷的含量为0.4%~0.6%。结论知母须根中酮成分含量较高,在知母须根的综合开发利用中可考虑此类成分。Objective To determine the content of neomangiferin,mangiferin,and isomangiferin in the fibrous root of Anemarrhena asphodeloides from different producing areas.Methods High-performance liquid chromatography with an ultraviolet detector was performed on a Shim-pack column(4.6 mm×250 mm,5μm)with a mobile phase of acetonitrile-0.4%glacial acetic acid for gradient elution at a flow rate of 1.0 mL/min,a column temperature of 25℃,and a detection wavelength of 258 nm.Results In the samples of the fibrous root of Anemarrhena asphodeloides,the content of neomangiferin,mangiferin,and isomangiferin was 1.2%-1.9%,1.0%-1.6%,and 0.4%-0.6%,respectively.Conclusion The content of xanthones is high in the fibrous root of Anemarrhena asphodeloides,which may be considered in the comprehensive exploitation and utilization of Anemarrhena asphodeloides.

关 键 词：产地 知母须根 口山酮 含量测定 

分 类 号：R927.2[医药卫生—药学]