Vaspin通过胰岛素信号通路改善地塞米松诱导的3T3-L1脂肪细胞的胰岛素抵抗  

Vaspin improves dexamethasone-induced insulin resistance of 3T3-L1 adipocytes viainsulin signaling pathway

作　　者：刘萍 农晰婷[2] 刘晓霞[2] 张薇[1] 武金娥 周鑫 成淑英[2] 孙超峰[3] LIU Ping;NONG Xi-ting;LIU Xiao-xia;ZHANG Wei;WU Jin-e;ZHOU Xin;CHENG Shu-ying;SUN Chao-feng(Department of Endocrinology,Xi’an Third Hospital,Xi’an 710018;Department of Endocrinology,Xi’an Central Hospital,Xi’an 710003;Department of Cardiology,the First Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710061,China)

机构地区：[1]西安市第三医院内分泌科,陕西西安710018 [2]西安市中心医院内分泌科,陕西西安710003 [3]西安交通大学第一附属医院心内科,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2018年第3期366-370,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：陕西省自然科学基础研究计划资助项目(No.2013JM4048)~~

摘　　要：目的探讨Vaspin对胰岛素抵抗脂肪细胞3T3-L1信号通路的作用及其机制。方法前脂肪细胞3T3-L1经地塞米松、IBMX及胰岛素混合诱导剂诱导8d,分化成为成熟脂肪细胞。(1)不同质量浓度Vaspin(0、50、100、200ng/mL)处理成熟3T3-L1细胞,观察PAKT/AKT通路的变化。(2)采用1μmol/L地塞米松建立胰岛素抵抗模型。分化成熟的脂肪细胞随机分为4组:对照组、模型对照组、实验组、Vaspin 200ng/mL+wortmannin 100nmol/L组。(3)观察Vaspin作用不同时间对胰岛素抵抗脂肪细胞葡萄糖消耗量的影响,Western blot分析胰岛素信号通路IRS-1、磷脂酰肌醇3激酶(PI3K)及Akt/phospho-Akt蛋白表达。结果 (1)Vaspin(0、50、100、200ng/mL)干预成熟脂肪细胞12h,Vaspin以浓度依赖的方式增加PAKT蛋白表达,与对照组相比,在100、200ng/mL组均有统计学差异(P<0.01)。(2)实验组葡萄糖消耗量均高于模型对照组(P<0.05);对照组与实验组比较,葡萄糖消耗量没有统计学差异(P>0.05)。(3)与对照组相比,模型对照组的IRS-1、PI3K、PAKT的蛋白表达低于对照组,差异有统计学意义(P<0.01);与模型对照组相比,实验组IRS-1、PI3K、PAKT的蛋白表达量均上调,差异有统计学意义(P<0.01);wortmannin预处理则阻断了Vaspin对PAKT/AKT、PI3K、IRS-1蛋白表达的上调。结论 Vaspin通过影响胰岛素抵抗的脂肪细胞中的PAKT/AKT胰岛素信号通路,增加葡萄糖的利用,改善了地塞米松诱导的3T3-L1脂肪细胞的胰岛素抵抗。3T3-L1 preadipocytes were induced for 8 days by mixed inducer of dexamethasone,IBMX and insulin.Then they were differentiated into mature adipocytes.①Different concentrations of Vaspin(0,50,100 and 200 ng/mL)were used to treat mature 3T3-L1 adipocytes and the changes in PAKT/AKT pathway were observed.②Dexamethasone of 1μmol/L was used to establish insulin resistance model;glucose oxidase method was used to measure glucose concentration in cell culture supernatant;the establishment of insulin resistance model was confirmed.③The effect of Vaspin on glucose consumption of insulin-resistant adipocytes during different time was observed;Western blotting test was used to analyze the expressions of insulin signaling pathway IRS-1,PI3K and Akt/phospho-Akt protein.Results ①We used Vaspin(0,50,100 and200 ng/mL)to treat mature adipocytes for 12 hours.Compared with that of the control,PAKT protein expression increased with Vaspin concentration;the difference was significant at concentrations of 100 ng/mL and 200 ng/mL(P<0.01).②Glucose consumption was higher in experimental Vaspin group than in model control group(P<0.05);there was no difference in glucose consumption between experimental Vaspin group and control group(P>0.05).③Compared with that in control group,protein expressions of IRS-1,PI3K and PAKT in model control group were significantly lower(P<0.01).Wortmannin pretreatment blocked protein expression upregulation of PAKT/AKT,PI3K and IRS-1 by Vaspin.Conclusion Vaspin increases uptake of glucose by insulin-resistant 3T3-L1 adipocytes through insulin signaling pathway and improving insulin resistance.

关 键 词：VASPIN 胰岛素抵抗 脂肪细胞 胰岛素信号通路 

分 类 号：R589.2[医药卫生—内分泌]