结核分枝杆菌PPE36蛋白增加耻垢分枝杆菌在小鼠巨噬细胞内生存及调节细胞因子的分泌  

Mycobacterium tuberculosis PPE36 protein expressed in Mycobacterium smegmatis enhances mycobacterial survival and modulates cytokine secretion in mouse macrophages

作　　者：梁丽娟 米友军 周明方 王雪林 LIANG Li-juan;MI You-jun;ZHOU Ming-fang;WANG Xue-lin(School of Clinical Medicine,Gansu University of Chinese Medicine,Lanzhou 730000;School of Basic Medicine,Lanzhou University,Lanzhou 730000;Lanzhou Pulmonary Hospital,Lanzhou 730000,China)

机构地区：[1]甘肃中医药大学临床医学院,甘肃兰州730000 [2]兰州大学基础医学院,甘肃兰州730000 [3]兰州市肺科医院,甘肃兰州730000

出　　处：《西安交通大学学报（医学版）》2018年第3期371-374,395,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：甘肃省高等学校科研项目(No.2017A-050);甘肃省自然科学基金科技计划项目(No.17JR5RA171)~~

摘　　要：目的研究结核分枝杆菌PPE36蛋白在宿主病原体相互作用时的功能。方法将结核分枝杆菌PPE36蛋白异源表达于非致病性的耻垢分枝杆菌MC2155,利用该重组菌感染小鼠巨噬细胞,分析重组菌在细胞内的生存、细胞死亡、细胞因子的变化。结果 PPE36能够增加耻垢分枝杆菌在小鼠巨噬细胞内的生存能力,诱导细胞的坏死,增加TNF-α、IL-1β、减少IL-6的表达。结论 PPE36表达于耻垢分枝杆菌之后能够促进细胞死亡,影响细胞因子分泌,增加在小鼠巨噬细胞内的持留。Objective To explore the function of Mycobacterium tuberculosis PPE36 protein in host-pathogen interactions.Methods The ppe36 gene was heterologously expressed in the nonpathogenic fast-growing Mycobacterium smegmatis MC 2155 strain.And we used this recombinant strain to infect mouse macrophages.Then we analyzed the strain s survival and cell death and change of cytokines within cells.Results PPE36 enhanced the intracellular survival of recombinant Mycobacterium smegmatis.Colonization of macrophages by MS-PPE36 was associated with cell death and significantly increased the expressions of TNF-αand IL-1βcompared with controls.Conclusion Our study indicates that PPE36 delivered in a live Mycobacterium smegmatis vehicle can promote host cell apoptosis,modify the cytokines profile of macrophages,and enhance the persistence of mycobacterium within mouse macrophages.

关 键 词：PPE36 结核分枝杆菌 巨噬细胞 

分 类 号：R378.91[医药卫生—病原生物学]