牛源犬新孢子虫孕鼠感染模型的建立  

Establishment of Model of Pregnant Mice Infected by Neospora caninumfrom Bovine

作　　者：梁阳 谢素珠 李航[1] 张宁宁 贾立军[1] LIANG Yang;XIE Suzhu;LI Hang;ZHANG Ningning;JIA Lijun(Department of Veterinary Medicine,Agriculture College of Yanbian University,Yanji 133002,China)

机构地区：[1]延边大学农学院动物医学系,延吉133002

出　　处：《中国畜牧兽医》2018年第4期1067-1073,共7页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(31760729;31360605);吉林省重点科技攻关项目(20140204078NY);吉林省自然科学基金(20160101201JC);吉林省教育厅2018年度"十三五"科研规划项目(JJKH20180905KJ)

摘　　要：为建立牛源犬新孢子虫孕鼠感染模型,深入研究牛源犬新孢子虫对孕鼠的致病作用,本试验以雌性BALB/c小鼠为试验动物,分离Vero细胞中培养的牛源犬新孢子虫速殖子,分不同剂量组腹腔接种雌性BALB/c小鼠后,与雄性BALB/c小鼠合笼,每天观察小鼠临床症状和发病情况,观察主要脏器组织的病理变化,应用PCR方法检测孕鼠脑、肝脏、脾脏等脏器组织及胎盘中犬新孢子虫Nc5基因,并测定孕鼠胎盘湿重和胎盘系数。结果显示,感染模型小鼠的最佳攻虫剂量为105个虫体;感染犬新孢子虫孕鼠先后出现精神不振、共济失调等临床症状,并有不同程度死亡;病理学观察模型小鼠脑、肝脏、脾脏等脏器组织出现充血、出血、肿大等病理变化;在模型小鼠脑、肝脏、脾脏等脏器组织及胎盘中检测到犬新孢子虫Nc5基因;随攻虫天数的增加,模型小鼠胎盘重量和胎鼠重量均不断增加,胎盘系数逐步降低,在第12、14、16天时,模型组与对照组相比,胎盘重量和胎盘系数均差异显著(P<0.05)。本试验成功建立了牛源犬新孢子虫孕鼠感染模型,为犬新孢子虫致病机制研究奠定了基础。To establish the model of pregnant mice infected by Neospora caninum(N.caninum)and understand the pathogenicity of N.caninum on pregnant mice,female BALB/c mice were inoculated intraperitoneally with different doses of N.caninum tachyzoites purified from Vero cells.Then these female BALB/c mice were maintained with male BALB/c mice in the same cage the next day.The clinical symptom and morbidity were observed daily.Basing on N.caninum Nc 5 gene,PCR was performed to detect parasitic DNA in brain,liver,spleen and placenta from N.canium-infected mice,placenta wet weight and placental coefficients of pregnant mice were measured,too.The results revealed that the optimal dose to infect mice was 10 5 tachyzoites.Pregnant mice behaved mental disorders,ataxia,and other clinical symptoms,even death simultaneously.Pathological changes such as congestion,hemorrhage,and enlargement occurred in the major organs of brain,liver,and spleen of model mice.Nc 5 gene was detected successively in brain,liver,spleen and placenta of pregnant mice infected with parasites.With the increase of post-infection days,the weight of placenta and the fetus were increasing and the placental coefficient decreased gradually following infection.Significant differences were observed in placental weight and placental coefficient between model group and control group on 12,14 and 16 d(P<0.05),respectively.The results indicated that the model of pregnant BALB/c mice infected by N.caninum was successfully established which laid the foundation for the study of pathogenesis of N.caninum.

关 键 词：牛 犬新孢子虫 孕鼠 模型 

分 类 号：S852.723[农业科学—基础兽医学]