丙泊酚对脂多糖诱导的人脐静脉内皮细胞p38 MPK/NF-κB信号通路活性的影响  

The effect of Propofol on the activation of p38 MAPK/NF-κB signal pathway in human umbilical vein endothelial cells induced by lipopolysaccharide

作　　者：汪鑫[1,2] 雷晓文 陈丹 刘斌 WANG Xin;LEI Xiao-wen;CHEN Dan;LIU Bin(Department of Anesthesia,Guizhou Provincial People′s Hospital,Guiyang 550002,China;Department of Anesthesia,West China Hospital of Sichuan University,Chengdu 610041,China)

机构地区：[1]贵州省人民医院麻醉科,贵州贵阳550002 [2]四川大学华西医院麻醉科,四川成都610041

出　　处：《中国当代医药》2018年第9期4-6,11,共4页China Modern Medicine

基　　金：贵州省科技厅联合基金项目(黔科合LH字[2015]7142号);贵州省科技厅软科学项目(黔科合基础[2016]1532-3);贵州省人民医院青年基金项目(GZSYQN[2015]09号)

摘　　要：目的研究丙泊酚对脂多糖(LPS)诱导的人脐静脉血管内皮细胞(HUBECs)p38 MAPK/NF-κB信号通路活性的影响。方法将体外培养的HUBECs随机分为4组:对照组(C组):给予脂肪乳20 mg/L;LPS组(L组):给予LPS 10 mg/L;丙泊酚组(P组):给予丙泊酚20 mg/L;丙泊酚+LPS组(PL组):给予丙泊酚20 mg/L、LPS 10 mg/L。在给予相应药物后1、2、3、6、12 h五个时间点检测p38 MAPK、p-p38 MAPK、NF-κB含量变化。结果予以给药1 h时,L组的p-p38 MAPK、NF-κB灰度值分别为10.32±0.73、60.32±5.73,与C组比较表达显著增加(P<0.05)。予以给药2 h时,L组的p-p38 MAPK、NF-κB灰度值分别为9.23±0.53、69.23±5.53,与C组比较,表达显著增加(P<0.05);PL组的p-p38 MAPK灰度值为6.32±0.35,与L组比较,显著降低(P<0.05)。给药3 h时,L组的p-p38 MAPK、NF-κB灰度值分别为11.13±0.23、71.13±5.23,与C组比较,表达显著增加(P<0.05);PL组的p-p38 MAPK、NF-κB灰度值分别为8.95±0.21、56.95±5.21,与L组比较,显著降低(P<0.05)。给药6 h时,L组的p-p38 MAPK、NF-κB灰度值分别为12.16±0.20、73.16±4.20,与C组比较,表达显著增加(P<0.05);PL组的p-p38 MAPK、NF-κB灰度值分别为9.65±0.32、58.65±4.32,与L组比较,显著降低(P<0.05)。给药12 h时,L组的p-p38 MAPK、NF-κB灰度值分别为18.19±0.31、91.19±6.31,与C组比较,表达显著增加(P<0.05),PL组的p-p38 MAPK、NF-κB灰度值分别为12.65±0.12、63.36±5.12,与L组比较,显著降低(P<0.05)。结论 LPS刺激HUBECs能引起p-p38 MAPK、NF-κB表达增加,予以丙泊酚后抑制此种增加,这可能是丙泊酚抗炎的作用机制之一。Objective To study the influence of Propofol on the activity of p38,MAPK/NF-κB signal pathway in human umbilical vein endothelial cells(HUBECs)induced by lipopolysaccharide(LPS).Methods HUBECs cultured in vitro were randomly divided into the 4 groups:control group(group C)was given Intralipid 20 mg/L,LPS group(L group)was given LPS 10 mg/L,Propofol group(P group)was given Propofol 20 mg/L,Propofol+LPS group(PL group)was given Propofol 20 mg/L and LPS 10 mg/L.The change in the content of p38 MAPK,p-p38 MAPK and NF-κB were detected at five time points of 1,2,3,6,and 12 h.Results When administered 1 h,the gray value of p-p38 MAPK and NF-κB in group L was 10.32±0.73,60.32±5.73 respectively,which increased significantly compared with group C(P<0.05).When administered 2 h,the gray value of p-p38 MAPK and NF-κB in group L was 9.23±0.53,69.23±5.53,which increased significantly compared with group C(P<0.05),and the gray value of PL group was 6.32±0.35,which was significantly lower than that of group L.Administration of 3 h,p-p38 MAPK,NF-κB gray value in group L was 11.13±0.23,71.13±5.23 respectively,which increased significantly compared with group C(P<0.05).p-p38 MAPK,NF-κB gray value was 8.95±0.21,56.95±5.21 respectively in group PL,which decreased significantly compared with group L(P<0.05).Administration of 6 h,p-p38 MAPK,NF-κB grayvalue was 12.16±0.20,73.16±4.20 respectively in group L,which increased significantly compared with group C(P<0.05).p-p38 MAPK,NF-κB gray value was 9.65±0.32,58.65±4.32 respectively in group PL,which decreased significantly compared with the L group(P<0.05).Administration of 12 h,p-p38 MAPK,NF-κB gray value was 18.19±0.31,91.19±6.31 respectively,which increased significantly compared with group C(P<0.05).p-p38 MAPK,NF-κB gray value in group PL was 12.65±0.12,63.36±5.12 respectively,which decreased significantly compared with group L(P<0.05).Conclusion LPS stimulating HUBECs can increase the expression of p-p38 MAPK and NF-κB,and inhibit this increase after Propof

关 键 词：丙泊酚 人脐静脉内皮细胞 炎症 

分 类 号：R614.24[医药卫生—麻醉学]