活血通络膏对佐剂性关节炎大鼠抗炎作用的实验研究  

Experimental Study of Anti-inflammatory Effect of Huoxue Tongluo Ointment on Adjuvant Arthritis Rats

作　　者：刘博华 马建辉 张玉华 曹东义 LIU Bo-hua;MA Jian-hui;ZHANG Yu-hua;CAO Dong-yi(Sports Science Research Institute of Hebei Province,Shijiazhuang 050011)

机构地区：[1]河北省体育科学研究所,石家庄050011 [2]河北省中医药科学院,石家庄050031

出　　处：《河北中医药学报》2018年第2期4-6,18,共4页Journal of Hebei Traditional Chinese Medicine and Pharmacology

基　　金：河北省中医药管理局科研计划项目:No.2013174

摘　　要：目的:研究活血通络膏对佐剂性关节炎(AA)大鼠的抗炎作用。方法:选取60只SD雌性大鼠随机分为正常组、模型组、阳性对照组(扶他林乳膏,2 g/次)、活血通络膏高、中、低剂量组(药物浓度分别为80%、40%、20%,5 g/次),每组10只大鼠,除正常组外,其它组采用弗氏完全佐剂0.1 m L左后足跖皮内注射制成AA大鼠模型,造模第9 d分别涂搽给药,每日2次,持续至第21 d。定点检测大鼠关节疼痛指数、关节炎指数、及血清骨桥蛋白(OPN)、前列腺素E2(PGE2)水平。结果:活血通络膏各剂量组和阳性对照组大鼠毛色、活动力等状况总体上优于模型对照组。与模型对照组比较,活血通络膏各剂量组大鼠关节疼痛指数、关节炎指数以及血清OPN、PGE2水平均降低,以高剂量组效果最好(P<0.05或P<0.01)。结论:活血通络膏明显缓解大鼠关节炎症,其作用机制可能与降低OPN、PGE2水平有关。Objective:To study the anti-inflammatory effect of Huoxue Tongluo ointment on adjuvant arthritis rats.Methods:60 SD female rats were randomly divided into the normal group,the model group,the positive control group(Votalin cream,2 g/time),the high,middle and low dose groups of Huoxue Tongluo(80%,40%,20%,5 g/time,respectively),each group of 10 rats;except the normal group,the other groups were given intradermal injections of Freund complete adjuvant 0.1 mL to the left posterior metatarsus to make the model of adjuvant arthritis rats.On the 9th day,the models were administered with inunction respectively,twice a day,till the 21st day.The joint pain index,arthritis index,serum osteopontin(OPN)and prostaglandin E2(PGE2)level were detected by fixed-point test.Results:Huoxue Tongluo ointment in each dose group and positive control group were better than model control group in terms of hair color and activity.Compared with the model control group,the joint pain index,arthritis index and serum OPN,PGE2 level in each dose group of Huoxue Tongluo ointment were all decreased,and the best effect was in the high dose group(P<0.05 or P<0.01).Conclusion:Huoxue Tongluo ointment significantly relieves arthritis in rats,and its mechanism may be related to lowering the level of OPN and PGE2.

关 键 词：活血通络膏 佐剂性关节炎 关节痛 经穴透皮疗法 骨桥蛋白 前列腺素E2 扶他林乳膏 

分 类 号：R285.5[医药卫生—中药学]