紫外线和热力对55型腺病毒的灭活效果  

Inactivation effect of UV and heat on adenovirus 55

作　　者：刘媛[1] 王文博[2] 邹自英[1] 邱薇[2] 范泉水[2] 冯子良[2] 熊杰[1] Liu Yuan;Wang Wenbo;Zou Ziying;Qiu Wei;Fan Quanshui;Feng Ziliang;Xiong Jie(Department of Laboratory,General Hospital of Chengdu Military Command,Chengdu,610083,China;The Center for Disease Prevention and Control,Chengdu Military Command,Chengdu,Sichuan,610031,China)

机构地区：[1]成都军区总医院检验科,成都610083 [2]成都军区疾病预防控制中心

出　　处：《西南国防医药》2018年第4期304-307,共4页Medical Journal of National Defending Forces in Southwest China

基　　金：国家自然科学基金青年项目(81301445);全军医学科技青年培育项目(14QNP057);上海市医学生物防护重点实验室开放课题(SKLM1401)

摘　　要：目的探讨紫外线和热力对55型腺病毒(HAdV-55)的灭活效果,为HAdV-55感染的防控提供参考。方法分别将HAdV-55置于波长245 nm的紫外线和65℃热力条件下作用30 min、1 h和2 h,通过定量PCR和病毒滴度试验,检测各处理因素对HAdV-55感染HEp-2细胞水平的影响。结果紫外线照射30 min后,HAdV-55感染HEp-2细胞的水平降低约50%;紫外照射1 h和2 h后,病毒仍具有一定感染能力,但感染水平显著降低。65℃热力处理30 min,HAdV-55感染HEp-2的水平降低了约104个数量级。紫外线和热力灭活的病毒产生子代病毒的能力均显著降低。结论紫外线和热力对HAdV-55都有灭活作用,其灭活作用的强弱与处理时间有关,254 nm紫外线照射和65℃热力处理30 min以上均能显著降低HAdV-55产生子代病毒的水平。Objective To explore the inactivation effects of UV and heat on human Adenovirus 55(HAdV-55)and provide references for prevention and control of HAdV-55 infection.MeAods HAdV-55 was placed under 245 nm UV and 65℃heat for 30 min,1 h and 2 h,respectively.Quantitative PCR and virus titer test were performed to examine the impacts of various factors on HAdV-55-infected HEp-2 cells.Results After 30 min's UV action,the level of HAdV-55-infected HEp-2 cells decreased by 50%approximately;after 1 h and 2 h's UV action,the virus was still infectious to some extent,but the level of infection decreased greatly.After 30 min's heat treatment at 65℃,the level of HAdV-55-infected HEp-2 cells decreased by about 104 orders of magnitude.Virus inactivated by UV and heat showed a significantly lowered capability of generating progeny virus.Conclusion Both UV and Heat can inactivate HAdV-55.The effect of inactivation depends on the time of action.More than 30 min's 254 nm UV action and heat treatment at 65℃can considerably reduce the capability of HAdV-55 to generate progeny virus.

关 键 词：紫外线 热力 灭活 腺病毒 

分 类 号：R446[医药卫生—诊断学]