海水和淡水养殖凡纳滨对虾肠道和鳃的菌群结构分析  

Microbial community structure analysis of intestine and gill of Litopenaeus vannamei in seawater and freshwater

作　　者：王元[1] 周俊芳[1] 韦信贤 马荣荣[1,3] 赵姝[1] 李新苍[1] 房文红[1] WANG Yuan;ZHOU Junfang;WEI Xinxian;MA Rongrong;ZHAO Shu;LI Xincang;FANG Wenhong(East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences/Key Laboratory of East China Sea Fishery Resources Exploitation,Ministry of Agriculture,Shanghai 200090,China;College of Aquatic and Life,Shanghai Ocean University,Shanghai 201306,China;Guangxi Academy of Fishery Science,Nanning,Guangxi 530021,China)

机构地区：[1]中国水产科学研究院东海水产研究所/农业部东海渔业资源开发利用重点实验室,上海200090 [2]广西水产科学研究院,广西南宁530021 [3]上海海洋大学水产与生命学院,上海201306

出　　处：《湖南农业大学学报（自然科学版）》2018年第2期198-203,共6页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：上海市科技兴农重点攻关项目(沪农科攻字(2014)第3–4号);上海市现代虾类产业技术体系专项(沪农科产字[2014]第5号)

摘　　要：运用Miseq高通量测序技术,分析凡纳滨对虾虾苗及其在海水和淡水2种养殖条件下生长到体长9~10 cm时的肠道和鳃的菌群结构。结果显示:虾苗、肠道和鳃样品在门水平上菌群均以厚壁菌门和变形菌门为主,但丰度差异较大;在属水平上,虾苗菌群以乳球菌属(36.1%)、未成功分属的γ–变形菌纲的菌株(25.5%)、芽孢杆菌属(13.5%)、Solibacillus(7.9%)、假单胞菌属(5.0%)和节杆菌属(2.5%)为主,鳃的菌群以乳球菌属、芽孢杆菌属、Solibacillus、假单胞菌属和节杆菌属为主,肠道菌群在海水养殖中的以发光杆菌属(22.9%)、弧菌属(18.2%)、乳球菌属(13.3%)、纤维弧菌属(8.0%)、希瓦氏菌属(5.5%)和芽孢杆菌属(5.4%)为主,而在淡水养殖中的以希瓦氏菌属(57.4%)、乳球菌属(18.8%)和芽孢杆菌属(6.3%)为主,弧菌丰度小于0.1%。养殖条件对凡纳滨对虾鳃中菌群结构影响较小,但对肠道菌群结构影响较大。The high-throughput Miseq sequencing technology was used to analyze the microbiota structure in Litopenaeus vannamei larvae,intestine and gill of young Litopenaeus vannamei under the condition of seawater and fresh water.The sequence results revealed that Firmicutes and Proteobacteria dominated in the larvae,intestines and gill with great variation in abundance.In particular,Lactococcus accounted for 36.1%in larvae microbiota community,Gammaproteobacteria_unclassified 25.5%,Bacillus 13.5%,Solibacillus 7.9%,Pseudomonas 5.0%and Arthrobacter 2.5%.In microbiota in gill tissue in both seawater and freshwater,Lactococcus,Bacillus,Solibacillus,Pseudomonas and Arthrobacter were dominant.Regarding to microbiota in intestine from seawater,Photobacterium accounted for 22.9%,Vibrio 18.2%,Lactococcus 13.3%,Cellvibrio 8.0%,Shewanella 5.5%,and Bacillus 5.4%,while in intestine from freshwater Shewanella 57.4%,Lactococcus 18.8%,Bacillus 6.3%,and Vibrio less than 0.1%.These results indicated that culture conditions had less influence on the microflora structure in gill than that while had great influence in intestine.

关 键 词：凡纳滨对虾 肠道 鳃 海水养殖 淡水养殖 菌群结构 高通量测序 

分 类 号：S917.1[农业科学—水产科学]