碘131标记壳聚糖/明胶-拓扑替康载药膜的制备、性能研究及SPECT/CT成像  

Preparation and Performance study of ^(131)I-labeled chitosan/gelatin-topotecan drug-loaded membrane

作　　者：周文彬 吕昂[2] 尹吉林[3] 王瑞敏 张金赫[3] 欧阳习[3] 李林娟 王欣璐[3] ZHOU Wenbin;LYU Ang;YIN Jilin;WANG Ruimin;ZHANG Jinhe;OUYANG Ai;LI Linjuan;WANG Xinlu(Pharmaceutical University,Guangdong pharmaceutical University,Guangzhou 510000,China;Nuclear Medicine Department,General Hospital of Guangzhou Military Command of PLA,Guangzhou 510000,China;College of Chemistry and Molecular Sciences,Wuhan University,Wuhan 430072,China)

机构地区：[1]广东药科大学基础学院,广州510000 [2]武汉大学化学与分子科学学院,武汉430072 [3]广州军区广州总医院核医学科,广州510000

出　　处：《功能材料》2018年第4期4144-4149,共6页Journal of Functional Materials

基　　金：国家杰出青年科学基金资助项目(50725416)

摘　　要：采用研究广泛的壳聚糖及明胶作为原材料,以京尼平作交联剂,制备具有良好生物相容性的壳聚糖/明胶复合膜。对该复合膜进行化疗药拓扑替康的负载以及放射性碘131标记,测试复合膜的含药量、药物释放、体外降解、碘标记率。把复合膜植入到KM小鼠腹腔中以探究复合膜的体内降解及生物相容性,并对该KM小鼠进行SPECT、CT成像。实验表明,复合膜已实现放化疗药物的同时载药;复合膜体外降解缓慢并且持续缓释药物;通过KM小鼠腹腔植入实验可知,复合膜具备可降解性的同时生物相容性良好。A chitosan/gelatin composite membrane with good biocompatibility was prepared by using widely studied chitosan and gelatin as raw materials with genipin as crosslink agent.The composite membrane was subjected to drug loading with topotecan and radioactive labeling with iodine-131,study its performance including drug content,drug release,in vitro degradation and labeling rate.KM mice were intraperitoneally implanted with composite membrane to explore the in vivo degradation and biocompatibility of the composite membrane,and the KM mice were subjected to SPECT and CT imaging.Experiments show that the composite membrane simultaneously achieves labeling with radiotherapy drug iodine-131 and drug loading with chemotherapy drug topotecan.In vitro degradation of the composite membrane was slow and drug release was sustained.Through the KM mice intraperitoneal implantation experiments,the composite membrane showed the in vivo biodegradability and good biocompatibility.

关 键 词：壳聚糖 明胶 碘标记 载药 生物安全 体内降解 

分 类 号：R318.08[医药卫生—生物医学工程]