电针对慢性应激抑郁大鼠血清6酮前列腺素F1α、血栓素B2、P-选择素水平的影响  

Impact of electro-acupuncture on serum 6-keto-PGF1α,TXB2 and P-selectin level of chronic stress depression rats

作　　者：刘俊彤[1] 李昱颉[1] 景泉凯 曹瑾[1] 杨利娟[1] 赵江豪 牟秋杰[1] 吕威[1] 赛音朝克图 李志刚[1] IU Juntong;LI Yujie;JING Quankai(School of Acupuncture and massage,Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学针灸推拿学院,硕士研究生100029 [2]内蒙古自治区国际蒙医医院五疗康复科

出　　处：《环球中医药》2018年第4期481-485,共5页Global Traditional Chinese Medicine

基　　金：国家自然科学基金地区科学基金(81360576);国家自然基金面上项目(81072860);内蒙古自治区科技厅自然科学基金项目(2015MS08130)

摘　　要：目的通过实验证明电针抗抑郁治疗是否会对慢性应激抑郁大鼠血浆中的6-ketoPGF1α、TXB2、P-selectin产生影响,并与药物治疗的效果相比较,从而进一步阐明抑郁症影响心血管疾病的相关的病理、生理学机制。方法成年雄性健康SD大鼠80只,饲养1周后通过开野实验将水平运动和垂直运动不足30次或超过120只的大鼠剔除,剩余32只随机分为空白组、模型组、药物组和电针组,每组8只。给予不可预知的应激刺激21天制作慢性不可预测应激抑郁模型。药物组灌胃给予百忧解,电针组取"百会""印堂"穴平刺进针进行刺激。22天后行开野实验、糖水消耗实验并断头取血,采用ELISA法测定血浆6-keto-PGF1α、TXB2、P-selectin含量,采用高效液相色谱法检测海马和皮层组织中5-HT、DA、NE的含量。结果造模后各组大鼠间相对糖水消耗量、水平运动和垂直运动结果均有显著性差异(P<0.05),模型组大鼠水平运动及垂直运动格数均显著低于空白组(P<0.05),电针组大鼠运动格数显著高于模型组(P<0.05)。各组大鼠海马组织中5-HT、DA、NE含量均有显著差异(P<0.05),其中模型组5-HT、DA、NE含量均显著低于空白组(P<0.05),药物组和电针组大鼠5-HT、DA、NE含量均显著高于模型组(P<0.05)。各组大鼠血浆中6-keto-PGF1α、TXB2、P-selectin含量均有统计学差异(P<0.05),其中药物组及电针组大鼠血浆中6-keto-PGF1α含量均显著高于模型组(P<0.05),TXB2、P-selectin含量均显著低于模型组(P<0.05)。结论电针治疗具有抗抑郁效果,可有效改善慢性应激抑郁大鼠的行为能力及海马组织中5-HT、DA、NE含量水平,并使其血浆中的6-keto-PGF1α含量升高,TXB2、P-selectin含量降低,进一步提示抑郁症可能通过影响6-keto-PGF1α/TXB2、P-selectin水平引起血小板活化,从而影响心血管疾病的发生及预后。Objective To prove the impact of antidepressive treatment of electroacupuncture on plasma 6-keto-PGF1α,TXB2 and P-selectin of chronic stress depression rats,and compare with the effects of drug treatment,so as to further clarify the pathophysiology mechanisms of depression effect on cardiovascular diseases.Methods 80 healthy adult male SD rats were selected.After 1 weeks of rearing,the rats were removed by open field experiment,horizontal movement and vertical movement less than 30 times and more than 120 times.The remaining 32 were randomly divided into blank group,model group,drug group and the electroacupuncture group,8 rats in each group.The rats were given unpredictable stress to induce depression model.Drug group was received gavage with prozac,electroacupuncture group was took“Baihui”,“Yin tang”as needle stimulation points.Open field experiment and sugar consumption experiments were conducted.Blood collection by decapitated after 22 days.Plasma 6-keto-PGF1α,TXB2,P-selectin were measured by ELISA method,the content of 5-HT,DA and NE in hippocampus and cortex tissue were detected by high performance liquid chromatography(HPLC)method.Results The results of sugar water consumption,horizontal movement and vertical movement of each group were significantly different(P<0.05).The number of horizontal motion and vertical movement in model group was significantly lower than that in blank group(P<0.05),and the number of movement in electroacupuncture group was significantly higher than that in model group(P<0.05).The content of 5-HT,DA and NE in hippocampus and cortex tissue of each group had significant difference(P<0.05).The contents of 5-HT,DA and NE in the model group were significantly lower than those in the blank group(P<0.05),and the contents of 5-HT,DA and NE in the drug group and electroacupuncture group were significantly higher than those in the model group(P<0.05).The levels of 6-keto-PGF1 alpha,TXB2 and P-selectin in plasma were statistically different(P<0.05).Among them,the content of 6-keto-

关 键 词：电针 慢性应激 抑郁 6酮前列腺素F1α 血栓素B2 P-选择素 

分 类 号：R245[医药卫生—针灸推拿学]