钝叶草盐胁迫的临界浓度初步研究  

Critical concentration of salt stress in Stenotaphrum secundatum

作　　者：徐毓皎 周宇杰 罗瑛 廖丽 白昌军[2] 王志勇 Xu Yu-jiao;Zhou Yu-jie;Luo Ying;Liao Li;Bai Chang-jun;Wang Zhi-yong(Institute of Tropical Agriculture and Forestry,Hainan University,Haikou 570228,Hainan,China;Tropical Pasture Research Center CATAS,Danzhou 571737,Hainan,China)

机构地区：[1]海南大学热带农林学院,海南海口570228 [2]中国热带农业科学院热带作物品种资源研究所,海南儋州571737

出　　处：《草业科学》2018年第4期816-821,共6页Pratacultural Science

基　　金：国家自然科学基金--中国钝叶草种质资源遗传多样性及重要性状的遗传标记研究(31560564)

摘　　要：本研究拟通过水培法筛选钝叶草(Stenotaphrum secundatum)盐胁迫半致死浓度,为后期筛选耐盐钝叶草品系提供依据。研究分别以0、35、70、105、140、175、210、245、280和315 mmol·L^(-1) 10个NaCl浓度梯度处理钝叶草,以叶色、枯黄率、坪用质量、匍匐茎长度和干物质重量5个指标进行综合评价,得出盐胁迫半致死临界浓度。结果表明,不同NaCl浓度处理下各指标之间存在显著差异(P<0.05)。随着处理盐浓度的增加,枯黄率显著下降(P<0.05),其他指标均呈显著上升趋势(P<0.05)。以钝叶草枯黄率数据建立线性回归方程,以50%枯黄率为标准,确定钝叶草的盐胁迫半致死临界浓度为185mmol·L^(-1),研究结果为今后钝叶草种质资源大量筛选提供依据。The objective of this study was to identify the semi-lethal concentrations of salt stress in Stenotaphrum secundatum using hydroponics to provide a theoretic background for screening salt tolerance of different accessions.The experiment was carried out at 10 concentration gradient treatments,which were 0,35,70,105,140,175,210,245,280,and 315 mmol·L-1.To investigate morphological indicators of S.secundatum,plants were treated after 28 days to determine the semi-lethal concentration of NaCl.The results showed that different treatments had significant differences(P<0.05).With the increase in the salt concentration of the treatment,four morphological indexes,which were leaf color,turf quality,stolon length,and dry matter weight increased significantly(P<0.05),whereas leaf firing decreased(P<0.05).Using a linear regression equation,the semi-lethal concentration was found to be 185 mmol·L-1,when the rate of leaf firing was 50%.These results can provide a scientific basis and direction for the later screening of S.secundatum germplasm.

关 键 词：钝叶草 盐胁迫 半致死临界浓度 枯黄率 坪用质量 生长速率 回归分析 

分 类 号：S540.34[农业科学—作物学] Q945.78[生物学—植物学]