内生真菌发酵液浸种对干旱胁迫下黑麦草种子萌发的影响  

Effect of seed soaking with Epichlo? fermentation broth on germination characteristics in perennial ryegrass under drought stress

作　　者：刘静[1] 周景乐 陈振江[1] 魏学凯[1] 李涛 李春杰[1] Liu Jing;Zhou Jing-le;Chen Zhen-jiang;Wei Xue-kai;Li Tao;Li Chun-jie(State Key Laboratory of Grassland Agro-ecosystems;Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture;College of Pastoral Agriculture Science and Technology, Lanzhou University,Lanzhou 730020,Gansu,China)

机构地区：[1]草地农业生态系统国家重点实验室,农业部草牧业创新重点实验室,兰州大学草地农业科技学院,甘肃兰州730020

出　　处：《草业科学》2018年第4期839-847,共9页Pratacultural Science

基　　金：国家重点基础研究发展规划(“973”计划)(2014CB138702);国家自然科学基金(31372366);长江学者和创新团队发展计划(IRT_17R50);中央高校基本科研业务费(lzujbky-2016-bt10、lzujbky-2017-kb10);111引智基地(B12002)

摘　　要：为了探究离体Epichlo?内生真菌对萌发期多年生黑麦草(Lolium perenne)种子抗旱性的影响。本研究以醉马草(Achnatherum inebrians)内生真菌(Epichlo3gansuensis)、披碱草(Elymus dahuricus)雀麦内生真菌(E.bromicola)和中华羊茅(Festuca sinensis)内生真菌(Epichlo?sp.)3种禾草内生真菌发酵液对多年生黑麦草种子进行浸种,采用纸上发芽法(TP)测定不同浓度PEG处理下种子萌发指标。结果表明:随PEG浓度的增大,黑麦草种子发芽率、发芽势、发芽指数和活力指数均逐渐降低,幼苗苗长和幼苗含水量逐渐减小,种子霉变率先增大后减小,根系长度均在-0.6 MPa时达到最大值。PEG浓度在^(-1).2 MPa时,种子萌发活力丧失。相与无浸种对照相比,内生真菌发酵液浸种可显著(P<0.05)促进黑麦草种子的萌发、幼苗和根的生长,有效降低种子的霉变率。醉马草内生真菌发酵液浸种下,多年生黑麦草种子发芽指数、活力指数、幼苗含水量、幼苗苗长和根系长度大于披碱草和中华羊茅浸种。综上所述,内生真菌发酵液对干旱胁迫下黑麦草种子的萌发具有一定的促进作用,且3种发酵液中,醉马草内生真菌发酵液抗旱效果比较明显。To explore the in vitro effect of Epichlo on the drought resistance in perennial ryegrass seeds during germination,the seeds were soaked in three species of Epichlo(E.gansuensis,E.bromicola,and Epichlo sp.)fermentation broth,to test the germination index of seeds under different concentrations of polyethylene glycol(PEG)through the paper germination test.The results showed that the seed germination rate,germination force,germination index,vigor index,seeding length,and seedling moisture content were decreased,the mildew rate was increased first and then decreased when the PEG concentration increased,and the root length of seedlings had a peak value at-0.6 MPa.The seeds inactivated and could not germinate when the concentration of PEG was-1.2 MPa.Compared to the control,soaking in Epichlo fermentation broth significantly(P<0.05)promoted seed germination,seedling growth,and root growth,and effectively reduced the mildew rate(P<0.05).Compared to seed soaking with Achnatherum inebrians Epichlo fermentation broth,the seed germination index,vigor index,seedling water content,seedling length,and root length of perennial ryegrass were greater than those in the Elymus dahuricus and Festuca sinensis experiments.In short,the fermentation broth of Epichlo had a certain effect on the germination of perennial ryegrass seeds under drought stress,and the effect of the A.inebrians fermentation broth was the strongest of the three different kinds of fermentation broth.

关 键 词：禾草内生真菌 浸种 萌发指标 霉变率 抗旱性 

分 类 号：S543.603.4[农业科学—作物学] Q945.78[生物学—植物学]