羊肚菌液体培养工艺研究  

Optimal Techniques for the Mycelium Liquid Culture Conditions of Morchella importuna

作　　者：李红[1] 张敏[1] 李超[1] 张季军[1] LI Hong;ZHANG Min;LI Chao;ZHANG Ji-jun(Edible Fungi Institute,Liaoning Academy of Agricultural Sciences,Shenyang 110161,China)

机构地区：[1]辽宁省农业科学院食用菌研究所,沈阳110161

出　　处：《沈阳农业大学学报》2018年第2期227-233,共7页Journal of Shenyang Agricultural University

基　　金：国家食用菌产业技术体系项目(CARS-20)

摘　　要：为获得人工栽培羊肚菌(Morchella)液体菌种生产的最佳工艺,以梯棱羊肚菌(Morchella importuna)菌株FY2为材料,通过单因素筛选不同培养温度、初始pH值、接种量、碳源、氮源对液体发酵的影响,根据单因素筛选结果采用四因素三水平的正交试验进行液体配方和发酵条件的优化,为工业化生产羊肚菌菌丝体相关产品的开发及液体菌种在栽培中的应用提供参考。单因素筛选结果表明:羊肚菌菌丝最适宜温度为25℃,菌丝体干重最大,显著高于其他温度处理;最适初始pH值为7,菌丝体干重最大;当接种量为5%和10%时,菌丝体干重都达到4.0g·L^(-1),差异不显著,所以综合成本考虑,确定最适接种量为5%;羊肚菌菌丝在葡萄糖、果糖、蔗糖、麦芽糖中菌丝体干重较大,达到5g·L^(-1)以上,但是差异不显著,综合成本考虑,选择葡萄糖做为羊肚菌菌丝生长的最佳碳源;羊肚菌菌丝在有机氮源中,黄豆粉生长的最好,有可能与黄豆粉是混合氮源,营养比较丰富有关,在无机氮源中,硝酸钾生长的最好,而这两种氮源中羊肚菌的菌丝体干重差异不显著,所以综合成本考虑,选择黄豆粉做为羊肚菌菌丝生长的最佳氮源。In this paper Morchella importuna'FY2'was used as a test material to obtain the optimal technology of Morhella liquid culture using single-factor test and orthogonal test L9(34).The results showed that the best growth temperature was 25℃for the mycelium of Morchella importuna,the best growth initial pH was 7.The mycelium dry weight could reach 4.0 g·L-1 when inoculating amounts were 5%and 10%.Therefore the best inoculating amount was 5%.The mycelium of Morchella importuna could grow in 7 carbon sources,the best carbon source is glucose.The mycelium of Morchella importuna can grow in 6 nitrogen sources,the best nitrogen source is soymeal.The effects of temperature and initial pH on the mycelium of Morchella importuna were very significant.The effects of carbon sources on the mycelium of Morchella importuna were significant.However,the effects of nitrogen sources on the mycelium of Morchella importuna were not significant.The optimal mycelium liquid culture conditions were temperature being 20℃,initial pH 7.0,glucose concentration 2.0%,soymeal concentration 0.15%.

关 键 词：羊肚菌 液体培养条件 正交试验 人工栽培 

分 类 号：S625.5[农业科学—园艺学]