体外释放酶免疫技术检测结核分枝杆菌的临床探讨  

Clinical Study on the Detection of Mycobacterium Tuberculosis by in Vitro Release Enzyme Immunoassay

作　　者：崔丽琳 CUI Lilin(Microbiological Laboratory,Dezhou City Decheng District Center for Disease Control and Prevention,Dezhou Shandong 253018,China)

机构地区：[1]德州市德城区疾病预防控制中心微生物检验科,山东德州253018

出　　处：《中国继续医学教育》2018年第12期41-42,共2页China Continuing Medical Education

摘　　要：目的对体外释放酶免疫技术检测结核分枝杆菌的临床应用价值进行探讨。方法将肺内结核、肺外结核、不明原因发热患者共90例以及健康志愿者30例作为研究对象,均采用体外释放酶免疫技术、胶体金法、痰涂片抗酸染色镜检以及聚合酶链式反应检测进行检测,对比四种检测方式的检测结果。结果体外释放酶免疫技术检测研究对象肺内结核、肺外结核、不明原因发热患者以及健康志愿者的灵敏度分别为74.29%、51.52%、63.64%和0.00%;胶体金法检测的灵敏度分别为45.71%、36.37%、36.37%和0.00%;痰涂片抗酸染色镜检测的灵敏度分别为37.14%、33.33%、18.18%和0.00%;聚合酶链式反应检测的灵敏度分别为20.00%、30.30%、13.63%和0.00%;体外释放酶免疫技术检测肺内结核、肺外结核、不明原因发热患者的灵敏度均高于其他三种检测方法,差异具有统计学意义(P<0.05)。结论体外释放酶免疫技术在检测结核分歧杆菌方面具有很好的敏感度及特异性,具有良好的应用价值。Objective To explore the clinical application value of the in vitro release enzyme immunoassay for the detection of Mycobacterium tuberculosis.Methods In this study,90 patients with pulmonary tuberculosis,extrapulmonary tuberculosis,unexplained fever and 30 healthy volunteers were tested with in vitro release enzyme immunoassay,colloidal gold,sputum smear,acid resistant staining and polymerase chain reaction.Results The sensitivity of extracorporeal release enzyme immunoassay was 74.29%,51.52%,63.64%and 0.00%in patients with pulmonary tuberculosis,extrapulmonary tuberculosis,unexplained fever and healthy volunteers.The sensitivity of colloidal gold method was 45.71%,36.37%,36.37%and 0.00%respectively.The sensitivity of sputum smear for acid fast staining was 37.14%,33.33%,18.18%and 0.00%respectively.The sensitivity of PCR was 20.00%,30.30%,13.63%and 0.00%respectively,the sensitivity of in vitro release enzyme immunoassay to detect pulmonary tuberculosis,extrapulmonary tuberculosis and fever of unknown origin was higher than that of the other three methods,and the difference was statistically significant(P<0.05).Conclusion The in vitro release enzyme immunoassay is good at the accuracy,in the detection of Mycobacterium tuberculosis in terms of sensitivity and specificity,has good application value,worthy of application and promotion.

关 键 词：体外释放酶免疫技术 结核分枝杆菌 临床应用价值 

分 类 号：R446[医药卫生—诊断学]